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Subject: Download: Real-Time Akt Pathway Monitoring for Oncology,
 Neuroscience, and Inflammatory Research
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30Gb8W2hy6Q-13q27kW97T-gT60VyPYf5-tmsd04 )

APPLICATION NOTE (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/M=
WBPkv9cp76W8b-n1l83HSKwW5l3Nw355D7MNN8wLL4F5m_5PW50kH_H6lZ3lsW5M5f0-1MhRgmW=
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y6Q-13q27kW97T-gT60VyPYf5-tmsd04 )

Download APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/MWBPkv9cp76W8b-n1l=
83HSKwW5l3Nw355D7MNN8wLL4F5m_5PW50kH_H6lZ3lsW5M5f0-1MhRgmW8rJ4Sq2GYTpjW2JMr=
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60VyPYf5-tmsd04 )

Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt act=
ivity in living cells

Cell signaling through the Akt pathway controls numerous key cellular funct=
ions including proliferation, survival, and migration. The ability to measu=
re real-time Akt activation in living cells will provide valuable informati=
on on the involvement of Akt across multiple fields of research including o=
ncology, neuroscience, and inflammatory disease research.

In this application note (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c=
3tLW04/MWBPkv9cp76W8b-n1l83HSKwW5l3Nw355D7MNN8wLL4F5m_5PW50kH_H6lZ3lsW5M5f0=
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N58JDk7k00k7W6nTJv43CZn1NW8JyvrM1Cf_LLW2_4tjP6DqgB9W8pt5-g8W5TB1W3bJYmV6n2S=
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30Gb8W2hy6Q-13q27kW97T-gT60VyPYf5-tmsd04 ) , we describe the use of Incucyt=
e=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt activity in livin=
g cells.

Topics include:

- Assay principle and quantification methods.
- Direct and indirect inhibition of Akt signaling in Glioblastoma.
- Targeting upstream inhibitors of Akt activity.
- Regulation of Akt activity in inflammation response.
- Akt activity and cell function regulation.

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et" data-hs-cos-type=3D"module"><table class=3D"hse-image-wrapper" role=3D"=
presentation" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" style=3D"b=
order-spacing:0 !important; border-collapse:collapse; mso-table-lspace:0pt;=
 mso-table-rspace:0pt">
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; mso-line-height-rule:exactly; font-family:Georgia, serif; color:#23286b; =
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MWBPkv9cp76W8b-n1l83HSKwW5l3Nw355D7MNN8wLL4F5nR32W50kH_H6lZ3n3N1sfyTdV3SK5W=
1hy-SJ6QsQHhV_-LnC7F6TszW5Fs3qR3Y11-yN6C9TGDCxw6hW22YbdT1CtB-CW6hcDtT83j5Qn=
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ame=3DSartorius%20-%20image-1.jpg" style=3D"outline:none; text-decoration:n=
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ge-1.jpg 600w, https://viewonline.labx.com/hs-fs/hubfs/Sartorius%20-%20imag=
e-1.jpg?upscale=3Dtrue&amp;width=3D2400&amp;upscale=3Dtrue&amp;name=3DSarto=
rius%20-%20image-1.jpg 1200w" sizes=3D"(max-width: 600px) 100vw, 600px">
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adding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#FFFFFF; padding-bottom:10px; =
padding-top:8px" bgcolor=3D"#FFFFFF">
    
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  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; pad=
ding:10px 20px"><div id=3D"hs_cos_wrapper_module_16239611830074" class=3D"h=
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lor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-genera=
l-type=3D"widget" data-hs-cos-type=3D"module">
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role=3D"presentation" style=3D"border-spacing:0 !important; mso-table-lspac=
e:0pt; mso-table-rspace:0pt; border-collapse:separate!important">
    <tbody><tr>
        
    
      <td align=3D"center" valign=3D"middle" bgcolor=3D"#D2232B" style=3D"b=
order-collapse:collapse; mso-line-height-rule:exactly; font-family:Georgia,=
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:20px; cursor:auto; background-color:#D2232B">
   
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
MWBPkv9cp76W8b-n1l83HSKwW5l3Nw355D7MNN8wLL4F5nR32W50kH_H6lZ3n0W8Chr7G5mgf2f=
W4FTfD41D0N1rW2QMDn_8b-RXKW8G99hY4pVWmWW53gh2C5Vf08MN4mxygTcT332W3t-45X8rqm=
-sV4ydl21Qs820W96PM6K4W3L2KW6h6PHP6ZQWvKN8_Zw4HcDzlSW4xwghR2XRYgNVNn6xh1134=
klN7Dkj-_XSJ-jW7-byFf3TStg5W5jb8xS4ZrmtrW4gnKCH6V6RG1W4kmKLv8620kFVWl9L23Vw=
xmYN8fXrb8dL5xNVqy3FK3Hyrx7N7fzCCck6RSpW3q0LXP8_GGwsW6lSfvn4Tthp3W4cRpnP7HN=
WyRW6l8jNH5GNmt_W3Dslxj6jpB7KVXPNxz1NKySjW7T75Sq6xbzBrVGTw1r28BZ7FW490KzM3K=
pKHgW7F7VgB4JqV6yf2gNKBK04" target=3D"_blank" style=3D"color:#00a4bd; mso-l=
ine-height-rule:exactly; font-size:22px; font-family:Arial, sans-serif; Mar=
gin:0; text-transform:none; text-decoration:none; padding:12px 18px; displa=
y:block" data-hs-link-id=3D"2">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">Download APPLICATION NOTE</strong>
        </a>
      </td>
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  </tbody></table>
</div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis=
 of Akt activity in living cells</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Cell signaling through the Akt pathway controls numerous key cellular=
 functions including proliferation, survival, and migration. The ability to=
 measure real-time Akt activation in living cells will provide valuable inf=
ormation on the involvement of Akt across multiple fields of research inclu=
ding oncology, neuroscience, and inflammatory disease research. </span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/MW=
BPkv9cp76W8b-n1l83HSKwW5l3Nw355D7MNN8wLL4F5nR32W50kH_H6lZ3m5VFH93x73WzzyW1F=
zyBq5hw6QYVsWwh824VvZCW5WkbYz8Y7VVHW6mX0cM7qHZFfVgygpw4tbTTkW5jP0k27qqP1ZW7=
9NYrR5t4kQ-W16NT264HqZpHN5hqGkvSnNrTW90CDH221spZyW5xSfKJ2QMGf7W6cNYCG8tLfx9=
W8kDlBt1vW8pHW3m-cNg8Qc8GcW29Br8L5Vp-kgW5sXm1J7nv6HvW7qhr9S3FLCcRW960xmT7jz=
SJqW8-R59v45VQhcW2P605R1_XZdbW6m4SxW39ZRqZW7__DdN3Ny8SSW3chngq27p9cHW7LPPDX=
4NgVW7W1yvyBl2DYCPjW8K-VPL3Wkt-KW72Tkgf2w9T8DVzlYMp92fdhqW93VlX03PtTryW7Xxt=
t-588KTTW3R0MRH3MSVnBf1qwmyT04" rel=3D"noopener" style=3D"mso-line-height-r=
ule:exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">In this=
 application note</a>, we describe the use of Incucyte=C2=AE Kinase Akt Len=
tivirus for kinetic analysis of Akt activity in living cells. </span></span=
></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Topics include: </span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Assay principle and quantification methods.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Direct and indirect inhibition of Akt signaling in Glioblastoma.</sp=
an></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Targeting upstream inhibitors of Akt activity.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Regulation of Akt activity in inflammation response.</span></span></=
li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Akt activity and cell function regulation.</span></span></li>
</ul></div></div></td></tr></tbody></table>
</div>


    
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