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Subject: App. Note: Real-Time Akt Pathway Monitoring for Oncology,
 Neuroscience, and Inflammatory Research
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_HYn7W5vCz384CKXMqW4q0Tn57mdNhBf8sfp0n04 )

APPLICATION NOTE (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/V=
WV7cB5fLknkW466xH-6cT5csW6fpr4J56v8QMN4gTlJ85m_5PW50kH_H6lZ3nFW8MCqBX1_zjv6=
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Cz384CKXMqW4q0Tn57mdNhBf8sfp0n04 )

Download APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VWV7cB5fLknkW466xH=
-6cT5csW6fpr4J56v8QMN4gTlJ85m_5PW50kH_H6lZ3nFW8MCqBX1_zjv6W6t-Mpf5GFLJZW1W3=
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CXt98-dfhwW8PzRwX8l3prvW6ZttbS5fgbq8W2Srtmc8crCszW1ysT_b6--KD8W7cvQZp5G2tf6=
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SSsW21H3F_3kllLsW1FX12V184bmfW1jm67m23_17NW27zhcv7_HYn7W5vCz384CKXMqW4q0Tn5=
7mdNhBf8sfp0n04 )

Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt act=
ivity in living cells

Cell signaling through the Akt pathway controls numerous key cellular funct=
ions including proliferation, survival, and migration. The ability to measu=
re real-time Akt activation in living cells will provide valuable informati=
on on the involvement of Akt across multiple fields of research including o=
ncology, neuroscience, and inflammatory disease research.

In this application note (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c=
3tLW04/VWV7cB5fLknkW466xH-6cT5csW6fpr4J56v8QMN4gTlJ85m_5PW50kH_H6lZ3nFW8MCq=
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W1B1XpN3CmJmGW2bW99B90x75hW5rz-Xr6bHwrHW7BPpWr7FSXbnW5l8MT18wsD1TN7bCnl6pb_=
QpW2lBMVb97tvffW1NRd_f7tsSSsW21H3F_3kllLsW1FX12V184bmfW1jm67m23_17NW27zhcv7=
_HYn7W5vCz384CKXMqW4q0Tn57mdNhBf8sfp0n04 ) , we describe the use of Incucyt=
e=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt activity in livin=
g cells.

Topics include:

- Assay principle and quantification methods.
- Direct and indirect inhibition of Akt signaling in Glioblastoma.
- Targeting upstream inhibitors of Akt activity.
- Regulation of Akt activity in inflammation response.
- Akt activity and cell function regulation.

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"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
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ding:10px 20px"><div id=3D"hs_cos_wrapper_module_16239611830074" class=3D"h=
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lor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-genera=
l-type=3D"widget" data-hs-cos-type=3D"module">
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role=3D"presentation" style=3D"border-spacing:0 !important; mso-table-lspac=
e:0pt; mso-table-rspace:0pt; border-collapse:separate!important">
    <tbody><tr>
        
    
      <td align=3D"center" valign=3D"middle" bgcolor=3D"#D2232B" style=3D"b=
order-collapse:collapse; mso-line-height-rule:exactly; font-family:Georgia,=
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:20px; cursor:auto; background-color:#D2232B">
   
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
VWV7cB5fLknkW466xH-6cT5csW6fpr4J56v8QMN4gTlJ85nR32W50kH_H6lZ3lzW6J_q_c2LR2d=
5W4z9sDf42QDRgW12mp2-92KbrGW3vsxJC7twjRWTvj_C2775JyW4BrrV2280zPCW5cy9D75MbN=
PGW3C73yD6bfdTDVgwHwR53bgMmVPmJhL6jhcH7W4_fSBW2whyjLW3R25HC67pW14V8nSB1934P=
jPVDcRzm48K6xsW62PCH_52VMgJVb-1FZ8qTf6MW3xkk8c7HFkShW85ZLFw2gPBd6W6whGhC5Hm=
PrCN6g5FTmlPkjFW6K9jvL7WSl_WW12NzpN8vYDTyW7_2pKR6FNW2RW61szjf2lw1K9W8m01PJ8=
4fXlPW4ycZpv836dVwW71y0tF3YMTMHW7wbV2k1CkXs4W6Wcf3D3yDfFSW2M0S_l4__YX5VD8dQ=
x1J_4DsW6jV1Q36WNwVlf8nNVGP04" target=3D"_blank" style=3D"color:#00a4bd; ms=
o-line-height-rule:exactly; font-size:22px; font-family:Arial, sans-serif; =
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play:block" data-hs-link-id=3D"2">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">Download APPLICATION NOTE</strong>
        </a>
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</div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis=
 of Akt activity in living cells</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Cell signaling through the Akt pathway controls numerous key cellular=
 functions including proliferation, survival, and migration. The ability to=
 measure real-time Akt activation in living cells will provide valuable inf=
ormation on the involvement of Akt across multiple fields of research inclu=
ding oncology, neuroscience, and inflammatory disease research. </span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VW=
V7cB5fLknkW466xH-6cT5csW6fpr4J56v8QMN4gTlJ85nR32W50kH_H6lZ3m3W560snn3W_ldZW=
3TmLZD46GwD9N41tkhTFvLpWN88CDm-4XJ0CW9gvtvd1Mz3XsW6BFnZq9k99j7VBVC7c6zR0-cW=
1YkLks6P52cNVg2Gd540-PTBVyxdc06zRS0MVV3Y6d3y25V-W6C-dXg2RcRN0W8c7mjS3_grRvW=
3FH6dB8crb3PW4y8jx02F1G2VW2TF3LM5BxZxvW3gQ0BL2dGzB9W795c_84YWNZ7VgDshg7vB2k=
qN2GQk-ZJtrDXW8WL1wf4NQ4wGW85RcCc10YK94W2L9ZB97j27NwW3YlMXw6lgyWtW7g6q2c2t6=
GPvW1tPv7N3nxQ2RW9b_7d64nH3tBN1Lc700GRs3lW6jZ7kb5g0DsbN2hTxyJ39jC6W8TC8CM7p=
DkgRW677jt87XgWFkf7sHjyF04" rel=3D"noopener" style=3D"mso-line-height-rule:=
exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">In this app=
lication note</a>, we describe the use of Incucyte=C2=AE Kinase Akt Lentivi=
rus for kinetic analysis of Akt activity in living cells. </span></span></p=
>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Topics include: </span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Assay principle and quantification methods.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Direct and indirect inhibition of Akt signaling in Glioblastoma.</sp=
an></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Targeting upstream inhibitors of Akt activity.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Regulation of Akt activity in inflammation response.</span></span></=
li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Akt activity and cell function regulation.</span></span></li>
</ul></div></div></td></tr></tbody></table>
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