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Subject: Unlock the Capabilities of Counterflow Centrifugation (CFC)
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Unlock the Capabilities of Counterflow Centrifugation (CFC)

Separation is a critical cell processing technique. For cell and gene thera=
py development and manufacturing, cell wash, concentration, and buffer exch=
ange are critically important techniques utilized throughout the cell proce=
ssing workflow.

Standard centrifugation is a well-known separation technique. However, the =
method presents challenges in cell processing due to increased risks from c=
ell stress, contamination, and scalability limitations. Alternatively, Coun=
terflow Centrifugation is an ideal technique due to its inherently gentle, =
low-shear environment, which enables the recovery of healthy and robust cel=
l fractions, while removing dead cells, resulting in high yields for downst=
ream processing.

This application note takes a detailed look at multiple parameters of CFC i=
ncluding:

- The basics of CFC
- The physics of fluidized beds
- Basic protocol steps and practical applications of CFC
- Automation-compatible media and reagents

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<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Unlock the Capabilities of Counterflow Centrifugation (CFC)</spa=
n></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Separation is a critical cell processing technique. For cell and gene=
 therapy development and manufacturing, cell wash, concentration, and buffe=
r exchange are critically important techniques utilized throughout the cell=
 processing workflow.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Standard centrifugation is a well-known separation technique. However=
, the method presents challenges in cell processing due to increased risks =
from cell stress, contamination, and scalability limitations. Alternatively=
, Counterflow Centrifugation is an ideal technique due to its inherently ge=
ntle, low-shear environment, which enables the recovery of healthy and robu=
st cell fractions, while removing dead cells, resulting in high yields for =
downstream processing.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%;=
 font-weight:bold"><span style=3D"color: #000000;"><span style=3D"font-fami=
ly: Arial, sans-serif;">This application note takes a detailed look at mult=
iple parameters of CFC including:</span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The basics of CFC</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The physics of fluidized beds</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Basic protocol steps and practical applications of CFC</span></span>=
</li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Automation-compatible media and reagents</span></span></li>
</ul></div></div></td></tr></tbody></table>
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