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Subject: Unlock an Advanced Solution for Monitoring Real-Time Akt Signaling
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APPLICATION NOTE (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/M=
VtBZHKNtt0W1Cgnqy8PCxwWW5b9Bvk59WmgNM_h9nv5m_5PW50kH_H6lZ3lhW1BdthX1g8nh8W5=
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_s50gb34W5lcs3V5pQR5Hf4cPrwY04 )

Download APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/MVtBZHKNtt0W1Cgnqy=
8PCxwWW5b9Bvk59WmgNM_h9nv5m_5PW50kH_H6lZ3lhW1BdthX1g8nh8W5-XYVj7CLwdtW9gBnx=
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QR5Hf4cPrwY04 )

Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt act=
ivity in living cells

Cell signaling through the Akt pathway controls numerous key cellular funct=
ions including proliferation, survival, and migration. The ability to measu=
re real-time Akt activation in living cells will provide valuable informati=
on on the involvement of Akt across multiple fields of research including o=
ncology, neuroscience, and inflammatory disease research.

In this application note (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c=
3tLW04/MVtBZHKNtt0W1Cgnqy8PCxwWW5b9Bvk59WmgNM_h9nv5m_5PW50kH_H6lZ3lhW1BdthX=
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9mM7CQTkdW3bjqz-7pGcqpW7NZ8hR6Q1K0fW8Vjytb6fmZcHW85L-C4878L5VW8s8j9k8y_122W=
4CbVQ68XL1YtW6FrPV67vzZfTW8bVtV35TmtrdW6rvM8P6CxvhzN7Y0cwGLrhK3W588Nrf56X7j=
BW6qKyZ55k2KVsW7XWpxf1Bvs-BW58gpR16wlHCFW8njYBZ6015TVW7fhgl71699Y4W8gPCF95H=
Pd3qVkNH_s50gb34W5lcs3V5pQR5Hf4cPrwY04 ) , we describe the use of Incucyte=
=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt activity in living=
 cells.

Topics include:

- Assay principle and quantification methods.
- Direct and indirect inhibition of Akt signaling in Glioblastoma.
- Targeting upstream inhibitors of Akt activity.
- Regulation of Akt activity in inflammation response.
- Akt activity and cell function regulation.

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order-spacing:0 !important; border-collapse:collapse; mso-table-lspace:0pt;=
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      <td class=3D"hs_padded" align=3D"left" valign=3D"top" style=3D"border=
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8CF-7m8PTnJTW5mYZWN7lcRhNW5k064D4FxBqQW1sTs6S8yd7mMW6XFmvT7FqBCyVf1DXm8z4fD=
XW7J0YmB4-YvRWV_MKQj23BvtpVD_cDZ17GB92W4SdCXk7xwBZJVsp_FT3Gg4HYN3ylmGfg7PQQ=
W4Nmpqz2XhVfBW1VMmnP3WJ9-6W4-DGm37JdQmsW3WgQJJ36__dtVSrPxv3DcFvsN1QvbQFVM1N=
qW6BvNCZ5vmYlSW951DF25NTV4sW1NXJd34M6Nm2VTLpZN57DmSSVJvkCH2HYYP5W2HpXSd5F1s=
XcW6pV3__3z72pdW30H1R-8WlbjnW1mHBSR6dlzrXW4xnwmG7W1MXgVJ-J0j28sW9sW5wh8W02X=
H20_W6Xfmzz42kxbSf1RrgWC04" target=3D"_blank" style=3D"color:#00a4bd; mso-l=
ine-height-rule:exactly" data-hs-link-id=3D"0">
        <img alt=3D"Link to APPLICATION NOTE" src=3D"https://viewonline.lab=
x.com/hs-fs/hubfs/1.1-%20All-Images/Allan%20Utton/labx%20red%20lgogo-png.pn=
g?width=3D300&amp;upscale=3Dtrue&amp;name=3Dlabx%20red%20lgogo-png.png" sty=
le=3D"outline:none; text-decoration:none; -ms-interpolation-mode:bicubic; b=
order:none; max-width:100%; font-size:16px" width=3D"150" align=3D"middle" =
srcset=3D"https://viewonline.labx.com/hs-fs/hubfs/1.1-%20All-Images/Allan%2=
0Utton/labx%20red%20lgogo-png.png?upscale=3Dtrue&amp;width=3D300&amp;upscal=
e=3Dtrue&amp;name=3Dlabx%20red%20lgogo-png.png 150w, https://viewonline.lab=
x.com/hs-fs/hubfs/1.1-%20All-Images/Allan%20Utton/labx%20red%20lgogo-png.pn=
g?upscale=3Dtrue&amp;width=3D600&amp;upscale=3Dtrue&amp;name=3Dlabx%20red%2=
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        </a>
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; Margin-left:auto; Margin-right:auto; border-collapse:collapse; bo=
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   =20
    
    

<div id=3D"column-1-0" class=3D"hse-column hse-size-12">
  <div id=3D"hs_cos_wrapper_module_16363849123814" class=3D"hs_cos_wrapper =
hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: inherit; =
font-size: inherit; line-height: inherit;" data-hs-cos-general-type=3D"widg=
et" data-hs-cos-type=3D"module"><table class=3D"hse-image-wrapper" role=3D"=
presentation" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" style=3D"b=
order-spacing:0 !important; border-collapse:collapse; mso-table-lspace:0pt;=
 mso-table-rspace:0pt">
  <tbody>
    <tr>
      <td align=3D"center" valign=3D"top" style=3D"border-collapse:collapse=
; mso-line-height-rule:exactly; font-family:Georgia, serif; color:#23286b; =
word-break:break-word; text-align:center; padding:0px; font-size:0px">
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
MVtBZHKNtt0W1Cgnqy8PCxwWW5b9Bvk59WmgNM_h9nv5nR32W50kH_H6lZ3nWW8h8tHv8GZGhsN=
2PN_xMyzslcW55bVrm78-8h7W31ng-z1p-6qnW79JWq36fm3CgVk-04F9jqZwDW3_V9VF22lSbf=
W3gzkSD1h9BRYW64-14x8JV9g4W8SzLBJ73v9S7W67SNRV59QzzzW31Kb-719HdPcN4C1rYVs41=
VhW5yNywN2pl358W46LfgN8F0Wm1W5DW2f97nclgBVY7bJj25Db4gW8lByZh5z0fyZW75NVnv1f=
3xv8W62l2zS7q_zl3W2Ms1Jm56YXl0W4WMQR-8t_7p_W1YBls02y9tThW7pMZTs3_L_0yW4LLCy=
559mFD1VVzG1260DjxHW4KDvsL4QtFv2W52RL_k4tkqPgW4xQWvQ1Gtv7cW1Btb-n6jtfMcV_75=
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so-line-height-rule:exactly" data-hs-link-id=3D"1">
        <img alt=3D"APPLICATION NOTE" src=3D"https://viewonline.labx.com/hs=
-fs/hubfs/Sartorius%20-%20image-1.jpg?width=3D1200&amp;upscale=3Dtrue&amp;n=
ame=3DSartorius%20-%20image-1.jpg" style=3D"outline:none; text-decoration:n=
one; -ms-interpolation-mode:bicubic; border:none; max-width:100%; font-size=
:16px" width=3D"600" align=3D"middle" class=3D"stretch-on-mobile" srcset=3D=
"https://viewonline.labx.com/hs-fs/hubfs/Sartorius%20-%20image-1.jpg?upscal=
e=3Dtrue&amp;width=3D1200&amp;upscale=3Dtrue&amp;name=3DSartorius%20-%20ima=
ge-1.jpg 600w, https://viewonline.labx.com/hs-fs/hubfs/Sartorius%20-%20imag=
e-1.jpg?upscale=3Dtrue&amp;width=3D2400&amp;upscale=3Dtrue&amp;name=3DSarto=
rius%20-%20image-1.jpg 1200w" sizes=3D"(max-width: 600px) 100vw, 600px">
        </a>
      </td>
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  </tbody>
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  <div id=3D"section-2" class=3D"hse-section" style=3D"padding-left:10px; p=
adding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; Margin-left:auto; Margin-right:auto; border-collapse:collapse; bo=
rder-spacing:0; background-color:#FFFFFF; padding-bottom:10px; padding-top:=
8px" bgcolor=3D"#FFFFFF">
    
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<div id=3D"column-2-0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; pad=
ding:10px 20px"><div id=3D"hs_cos_wrapper_module_16239611830074" class=3D"h=
s_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"co=
lor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-genera=
l-type=3D"widget" data-hs-cos-type=3D"module">
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  <table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" =
role=3D"presentation" style=3D"border-spacing:0 !important; mso-table-lspac=
e:0pt; mso-table-rspace:0pt; border-collapse:separate!important">
    <tbody><tr>
        
    
      <td align=3D"center" valign=3D"middle" bgcolor=3D"#D2232B" style=3D"b=
order-collapse:collapse; mso-line-height-rule:exactly; font-family:Georgia,=
 serif; font-size:18px; color:#23286b; word-break:break-word; border-radius=
:20px; cursor:auto; background-color:#D2232B">
   
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
MVtBZHKNtt0W1Cgnqy8PCxwWW5b9Bvk59WmgNM_h9nv5nR32W50kH_H6lZ3lhW50KfvN24-jFYW=
3BB83b10BC7HW72bCyy8Jy5dZW7wHM5V5jlGz8W1mP_1j3FVtr6N52RP94zck4WW6P6vGn7MW5M=
9W59-tpx2ThWG-W4JHMMZ4bFNm1W2zxWMf8VRC2WW51_f9t4QVf_RW5cZ9lG4rXGWqW6Sn58c49=
Vz1fW7wJscp8XGBChW4hX6m93-GFkMW4v21gF1Bt4_1W5y5vLM1tSW2cW6R0rV02jrJ4QW18zS5=
J1CDq7tW3MXBPr6p-9_rVS1H1L3dyCtsW56XRww69-wKCW5FcWVq3q1y_RV-t1hS4STxvFW1YZM=
rt3XFpKCW6nBSNV5SFrm4VbyXKr7Rm0bcW5wTVb98yXdqYN3HcP8t2fyk0W4wBW7z2bXtBdW66L=
3BF7HkG8cW7fLfmT2dG8vVf3kNKDP04" target=3D"_blank" style=3D"color:#00a4bd; =
mso-line-height-rule:exactly; font-size:22px; font-family:Arial, sans-serif=
; Margin:0; text-transform:none; text-decoration:none; padding:12px 18px; d=
isplay:block" data-hs-link-id=3D"2">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">Download APPLICATION NOTE</strong>
        </a>
      </td>
    </tr>
  </tbody></table>
</div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis=
 of Akt activity in living cells</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Cell signaling through the Akt pathway controls numerous key cellular=
 functions including proliferation, survival, and migration. The ability to=
 measure real-time Akt activation in living cells will provide valuable inf=
ormation on the involvement of Akt across multiple fields of research inclu=
ding oncology, neuroscience, and inflammatory disease research. </span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/MV=
tBZHKNtt0W1Cgnqy8PCxwWW5b9Bvk59WmgNM_h9nv5nR32W50kH_H6lZ3lBW1lfjRg89XXC7W8V=
pL3n2WbvGzW6QGnr-70B90nW40Bp4f3NMZ-3W2JQ3wt1qbrpTW3x8dqK6253tRN2D2wgw46Cd-W=
11k3KJ6YWg06W4dHrKV5tQdwCW8xnz9172DJN5W733zD_5HYkmtW32pwnD4xw98_W6cfFrF66V8=
QJVy_44Y615rNJW4J0zZZ4YCSwjW3Vw4305XLjLyW1zW98755DGLSW4QVNN284wN84W1rxRVx6T=
zs2JV2Z83j1fWy-KVZwQTg79VSBhW8yw90R1cLtjtF8Dhd1BQ_KJW2pYYFs5DL89_W3qsdP41pk=
G2nW68n56p3jCwjHW4ztHc198v0x5W3W99PV6JFQCqW7SZL237yq6PgW5qvMYb3TgwGSW3yB3CY=
2T4H-7N2yPNVcnMLqHf5sM5Pj04" rel=3D"noopener" style=3D"mso-line-height-rule=
:exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">In this ap=
plication note</a>, we describe the use of Incucyte=C2=AE Kinase Akt Lentiv=
irus for kinetic analysis of Akt activity in living cells. </span></span></=
p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Topics include: </span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Assay principle and quantification methods.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Direct and indirect inhibition of Akt signaling in Glioblastoma.</sp=
an></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Targeting upstream inhibitors of Akt activity.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Regulation of Akt activity in inflammation response.</span></span></=
li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Akt activity and cell function regulation.</span></span></li>
</ul></div></div></td></tr></tbody></table>
</div>


    
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