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2R7xW6vtxzN1ndPz5W3jVgZH8sRr0sdSmMC804 )

APPLICATION NOTE (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/V=
VqtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85m_5PW50kH_H6lZ3l2N25ClpsmL871W=
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xzN1ndPz5W3jVgZH8sRr0sdSmMC804 )

Download APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VVqtTw5q2mRQW21X2l=
y8-bvGyW6fpr4J5b9y63N1k24X85m_5PW50kH_H6lZ3l2N25ClpsmL871W2bCkhk5SzG1hW1_GS=
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sRr0sdSmMC804 )

Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt act=
ivity in living cells

Cell signaling through the Akt pathway controls numerous key cellular funct=
ions including proliferation, survival, and migration. The ability to measu=
re real-time Akt activation in living cells will provide valuable informati=
on on the involvement of Akt across multiple fields of research including o=
ncology, neuroscience, and inflammatory disease research.

In this application note (https://email.labxmediagroup.com/e3t/Ctc/LT+113/c=
3tLW04/VVqtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85m_5PW50kH_H6lZ3l2N25Cl=
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MW6CnkLw2F2C75W37-m5s416pX8W5N_HVQ2yJ7t-W1KFNhZ4KT_jdW6HQjRj8-RstMW1pT43z7j=
2R7xW6vtxzN1ndPz5W3jVgZH8sRr0sdSmMC804 ) , we describe the use of Incucyte=
=C2=AE Kinase Akt Lentivirus for kinetic analysis of Akt activity in living=
 cells.

Topics include:

- Assay principle and quantification methods.
- Direct and indirect inhibition of Akt signaling in Glioblastoma.
- Targeting upstream inhibitors of Akt activity.
- Regulation of Akt activity in inflammation response.
- Akt activity and cell function regulation.

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      <td class=3D"hs_padded" align=3D"left" valign=3D"top" style=3D"border=
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VVqtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85nR32W50kH_H6lZ3msW8pxxt65vDXx=
2W3CQPXf1jMPB3W7wTL6t3xkDqbW4HrWqY310z-gW6XcfbX6fMpCDW2NjKPL1J5F5TW9lXxQf8s=
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sdk1725bxW4czWBy297RdNW5zMV2S37VcJJW844PmK2S8W5HW88dV9H4CLLcpN1DSq7xTFcxDN2=
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g?width=3D300&amp;upscale=3Dtrue&amp;name=3Dlabx%20red%20lgogo-png.png" sty=
le=3D"outline:none; text-decoration:none; -ms-interpolation-mode:bicubic; b=
order:none; max-width:100%; font-size:16px" width=3D"150" align=3D"middle" =
srcset=3D"https://viewonline.labx.com/hs-fs/hubfs/1.1-%20All-Images/Allan%2=
0Utton/labx%20red%20lgogo-png.png?upscale=3Dtrue&amp;width=3D300&amp;upscal=
e=3Dtrue&amp;name=3Dlabx%20red%20lgogo-png.png 150w, https://viewonline.lab=
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        </a>
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hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: inherit; =
font-size: inherit; line-height: inherit;" data-hs-cos-general-type=3D"widg=
et" data-hs-cos-type=3D"module"><table class=3D"hse-image-wrapper" role=3D"=
presentation" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" style=3D"b=
order-spacing:0 !important; border-collapse:collapse; mso-table-lspace:0pt;=
 mso-table-rspace:0pt">
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    <tr>
      <td align=3D"center" valign=3D"top" style=3D"border-collapse:collapse=
; mso-line-height-rule:exactly; font-family:Georgia, serif; color:#23286b; =
word-break:break-word; text-align:center; padding:0px; font-size:0px">
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
VVqtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85nR32W50kH_H6lZ3nyW64MLP-2F5qH=
8MmdzTlJFJgxW8R6F4y6d9VM4W71q3Jf68d1NSW5Pwrw_3mgWcQVsNcyF4Hf3SlW4c6SqL52frw=
kW1QVzv44xF03ZW4gCxx_8_1X-XW2CZVsS3bRX_BW2PT1R28CPnrDW43qvDt4HLnkZVRDjNS85t=
rL8N6HTX6gGhRzbN9d8p_J6Km4FW8fL_zj3K8BrQW7L5FJX7jfr10W56324P6wDRWVVVVT7L1P9=
BV4VwfF_33McXFnW1Lv--W6vWHmkVbxc5M1-cQ3yW3ZLd8w2-MXMGVkD5cT2cBQtQW2ZcbRx10r=
FBNW2l0K0F8VBPbfW5sbs3z5lg0jsW7MSpfq42DswdW8n7ZYr3YjYQ0W64VJmK8fjBRdW7FmgxP=
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line-height-rule:exactly" data-hs-link-id=3D"1">
        <img alt=3D"APPLICATION NOTE" src=3D"https://viewonline.labx.com/hs=
-fs/hubfs/Sartorius%20-%20image-1.jpg?width=3D1200&amp;upscale=3Dtrue&amp;n=
ame=3DSartorius%20-%20image-1.jpg" style=3D"outline:none; text-decoration:n=
one; -ms-interpolation-mode:bicubic; border:none; max-width:100%; font-size=
:16px" width=3D"600" align=3D"middle" class=3D"stretch-on-mobile" srcset=3D=
"https://viewonline.labx.com/hs-fs/hubfs/Sartorius%20-%20image-1.jpg?upscal=
e=3Dtrue&amp;width=3D1200&amp;upscale=3Dtrue&amp;name=3DSartorius%20-%20ima=
ge-1.jpg 600w, https://viewonline.labx.com/hs-fs/hubfs/Sartorius%20-%20imag=
e-1.jpg?upscale=3Dtrue&amp;width=3D2400&amp;upscale=3Dtrue&amp;name=3DSarto=
rius%20-%20image-1.jpg 1200w" sizes=3D"(max-width: 600px) 100vw, 600px">
        </a>
      </td>
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adding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; Margin-left:auto; Margin-right:auto; border-collapse:collapse; bo=
rder-spacing:0; background-color:#FFFFFF; padding-bottom:10px; padding-top:=
8px" bgcolor=3D"#FFFFFF">
    
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<div id=3D"column-2-0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; pad=
ding:10px 20px"><div id=3D"hs_cos_wrapper_module_16239611830074" class=3D"h=
s_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"co=
lor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-genera=
l-type=3D"widget" data-hs-cos-type=3D"module">
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  <table align=3D"center" border=3D"0" cellpadding=3D"0" cellspacing=3D"0" =
role=3D"presentation" style=3D"border-spacing:0 !important; mso-table-lspac=
e:0pt; mso-table-rspace:0pt; border-collapse:separate!important">
    <tbody><tr>
        
    
      <td align=3D"center" valign=3D"middle" bgcolor=3D"#D2232B" style=3D"b=
order-collapse:collapse; mso-line-height-rule:exactly; font-family:Georgia,=
 serif; font-size:18px; color:#23286b; word-break:break-word; border-radius=
:20px; cursor:auto; background-color:#D2232B">
   
        <a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/=
VVqtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85nR32W50kH_H6lZ3mPW5H99Jg3FKlz=
2N911MrXdzBC7VS_8pG6Z4msYW2qsfnW1g7x18W8ykKKT4Z_VMPW91KFK91Ff-12N2_W1bmrH7_=
QW3WWSt37fhglxW980npY5j4wH3W6nKjkz3wjCthW3fdKK55WxKF_M6pJWkLR07cW42L2jw8cWl=
jnW3bdK8D78NR7YW7bcm0f8QQ6VnW8_sZP-4shqvWW3MNjB52fH75gW7BwTF51MJS8MMC_6Spfr=
P69W5hg5KV3qh8DFN2SgJCXZYt0tW5w-Ltf1BbFV4W4QJL5J2mkbZYW1D6HcJ83nyPmW1GkMp12=
VGrVgVkxD1k3qMCGtW5bWh0N8kBbkZW8yG7wQ6DNQj0W3g4Xhy5BDVRFW6pGhVM7GyZNGW8LTDW=
-3CBw6VW6FpJdR4NMfqqf4MG2V604" target=3D"_blank" style=3D"color:#00a4bd; ms=
o-line-height-rule:exactly; font-size:22px; font-family:Arial, sans-serif; =
Margin:0; text-transform:none; text-decoration:none; padding:12px 18px; dis=
play:block" data-hs-link-id=3D"2">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">Download APPLICATION NOTE</strong>
        </a>
      </td>
    </tr>
  </tbody></table>
</div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Use of Incucyte=C2=AE Kinase Akt Lentivirus for kinetic analysis=
 of Akt activity in living cells</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Cell signaling through the Akt pathway controls numerous key cellular=
 functions including proliferation, survival, and migration. The ability to=
 measure real-time Akt activation in living cells will provide valuable inf=
ormation on the involvement of Akt across multiple fields of research inclu=
ding oncology, neuroscience, and inflammatory disease research. </span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VV=
qtTw5q2mRQW21X2ly8-bvGyW6fpr4J5b9y63N1k24X85nR32W50kH_H6lZ3ppW9fgnyp7ksPCMW=
2sqdkQ42ZJqjW20t4FZ1HMqmqW6wdn2C7VYSp6VzDcQV7V2bFHW2D_wq-5GPW9fW5mBrB0684gQ=
VW78y79w5HXSbdMMCxphPlKdnW1zBkRX3jXrSWW7YgbpH6QvSq4W1r4sWh7Kp5b8V7RVBB8GtcC=
FVsssb228HmNcW3tkbxb2bvWkCW20Fybj1bX85kW2s5CHm7fxnN0W4Jm4q68YfXXnN5-vXk8nkx=
HWW4gbrDr53h-LlW5tGnwK57MpNlW7XXGrr2m-1TKW5l8CbL3q79kNW5nfQ2L1vN8f_W5_d8wq6=
dD9KHW90wJM97JDDJHW2W7gkN8zwLl1W2gjv5h73H_lPW8XyvFS4QxLH4W3MVRqG7LY3p_W8Hyl=
yX8qrTsZW2w86Wv1PXdWGf98ftW404" rel=3D"noopener" style=3D"mso-line-height-r=
ule:exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">In this=
 application note</a>, we describe the use of Incucyte=C2=AE Kinase Akt Len=
tivirus for kinetic analysis of Akt activity in living cells. </span></span=
></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Topics include: </span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Assay principle and quantification methods.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Direct and indirect inhibition of Akt signaling in Glioblastoma.</sp=
an></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Targeting upstream inhibitors of Akt activity.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Regulation of Akt activity in inflammation response.</span></span></=
li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Akt activity and cell function regulation.</span></span></li>
</ul></div></div></td></tr></tbody></table>
</div>


    
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ont-family:Geneva, Verdana, Arial, Helvetica, sans-serif; text-align:center=
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