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Subject: Application Note: A powerful image-based profiling tool!
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A powerful image-based profiling tool for the development of drug discovery=
 assays and multiparameter screening campaigns

Cell painting can be used to extract approximately 1,500 measurements from =
each cell based on changes in size, shape, texture, and fluorescence intens=
ity. These measurements can be used to detect subtle changes in phenotype a=
nd gain data-rich information on important cellular parameters.

Conventional high-throughput screening applications can evaluate only a few=
 measurements and are limited to compound-induced cellular changes. Cell pa=
inting, on the other hand, can be implemented in a multitude of diverse, hi=
gh-resolution applications including:

- Organelle-based measurements including that of the nucleus, endoplasmic r=
eticulum, mitochondria, and others.
- Single-cell measurements of cells most sensitive to compound exposure com=
pared with control cells.
- Identification of initial hits and mechanistic insights unreachable throu=
gh traditional high-throughput assays.

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<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">A powerful image-based profiling tool for the development of dru=
g discovery assays and multiparameter screening campaigns</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Cell painting can be used to extract approximately 1,500 measurements=
 from each cell based on changes in size, shape, texture, and fluorescence =
intensity. These measurements can be used to detect subtle changes in pheno=
type and gain data-rich information on important cellular parameters.</span=
></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Conventional high-throughput screening applications can evaluate only=
 a few measurements and are limited to compound-induced cellular changes. C=
ell painting, on the other hand, can be implemented in a multitude of diver=
se, high-resolution applications including:</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Organelle-based measurements including that of the nucleus, endoplas=
mic reticulum, mitochondria, and others.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Single-cell measurements of cells most sensitive to compound exposur=
e compared with control cells.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Identification of initial hits and mechanistic insights unreachable =
through traditional high-throughput assays.</span></span></li>
</ul></div></div></td></tr></tbody></table>
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