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✏️ Editing: 1678458796.M361915P416094.aristoteles.x5servers.net,S=62832,W=64388:2,

Envelope-to: rcruz@sapasequipment.com
Delivery-date: Fri, 10 Mar 2023 10:33:16 -0400
Date: Fri, 10 Mar 2023 08:32:32 -0600 (CST)
From: Sartorius <news@sartorius.com>
Reply-To: news@sartorius.com
To: rcruz@sapasequipment.com
Message-ID: <2122011821.24511860.1678458752921@nld1mktmail-batch1d.marketo.org>
Subject: Latest App Note: Screening and Characterizing mAbs
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Characterizing mAbs Using Advanced Flow Cytometry and BLI Label-free Detect=
ion
New application note
Continued growth and diversification of the monoclonal antibody (mAb) thera=
peutic market requires reliable and robust techniques for the profiling and=
 screening of drug candidates. This is approached using the combined Octet=
=C2=AE BLI Label-Free Detection and iQue=C2=AE Advanced Flow Cytometry plat=
forms. Octet evaluates post-translational modifications while the iQue=C2=
=AE delivers live cell-based assessment of binding. This application note s=
hows a range of experiments displaying the power of leveraging these instru=
ments to provide broad assessment of novel mAbs to advance drug discovery w=
orkflows.

Key Takeaways:
Assess binding and specificity on live cells
Identify mAbs that compete for epitope binding
Rank antibodies based on glycosylation status
Analyze Fc receptor function in immune cell co-culture
Discover a robust and high-throughput method for screening and characterizi=
ng mAb drug candidates

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<tr>=20
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eight: 20px; font-size: 0px; line-height: 0px;" height=3D"20" align=3D"left=
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</tr>=20
</tbody>=20
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<table class=3D"mktoModule globalModule headline headlineOnWhite" id=3D"h1O=
nWhite87ee8e4a-2855-4b0e-ad54-81f15bea5dda" style=3D"border: none; border-c=
ollapse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; rol=
e: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 100%;=
 background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspacing=
=3D"0" role=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<td class=3D" mktoText globalModuleInner globalText globalH1 headlineText "=
 id=3D"h1OnWhiteText87ee8e4a-2855-4b0e-ad54-81f15bea5dda" style=3D"width: 5=
60px; font-family: Arial, sans-serif; font-weight: normal; mso-line-height-=
rule: exactly; text-align: left; color: #000000; vertical-align: top; font-=
size: 30px; line-height: 36px;" width=3D"560" align=3D"left" valign=3D"top"=
>Characterizing mAbs Using Advanced Flow Cytometry and BLI Label-free Detec=
tion</td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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</tr>=20
<tr>=20
<td class=3D"headlineSpacerBottom" colspan=3D"3" height=3D"20px" style=3D"t=
ext-align: left; vertical-align: top; font-size: 0px; line-height: 0px; hei=
ght: 20px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
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<table class=3D"mktoModule globalModule headline headlineOnWhite" id=3D"h2O=
nWhiteab0c1cf9-e14d-4280-b7b9-4042c5bf83c1" style=3D"border: none; border-c=
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 background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspacing=
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<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<td class=3D" mktoText globalModuleInner globalText globalH2 headlineText "=
 id=3D"h2OnWhiteTextdfb9be71-5a04-4f59-a237-3cbc0f9942fc" style=3D"width: 5=
60px; font-family: Arial, sans-serif; font-weight: normal; text-align: left=
; color: #000000; vertical-align: top; font-size: 22px; line-height: 26px;"=
 width=3D"560" align=3D"left" valign=3D"top">New application note</td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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</tr>=20
<tr>=20
<td class=3D"headlineSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"t=
ext-align: left; vertical-align: top; font-size: 0px; line-height: 0px; hei=
ght: 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
</table>
<table class=3D"mktoModule globalModule text textOnWhite" id=3D"textOnWhite=
5aabd0d7-ab33-4a40-b357-dba164bae11e" style=3D"border: none; border-collaps=
e: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; role: pre=
sentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 100%; backg=
round-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" r=
ole=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<td class=3D"mktoText globalModuleInner globalText textText " id=3D"textOnW=
hiteText16761a73-2a5a-44fb-9d6d-00590be39256" style=3D"width: 560px; font-f=
amily: Arial, sans-serif; font-weight: normal; font-size: 15px; line-height=
: 26px; text-align: left; color: #000000; vertical-align: top;" width=3D"56=
0" align=3D"left" valign=3D"top">Continued growth and diversification of th=
e monoclonal antibody (mAb) therapeutic market requires reliable and robust=
 techniques for the profiling and screening of drug candidates. This is app=
roached using the combined Octet<sup>=C2=AE</sup> BLI Label-Free Detection =
and iQue<sup>=C2=AE</sup> Advanced Flow Cytometry platforms. Octet evaluate=
s post-translational modifications while the iQue<sup>=C2=AE</sup> delivers=
 live cell-based assessment of binding. This application note shows a range=
 of experiments displaying the power of leveraging these instruments to pro=
vide broad assessment of novel mAbs to advance drug discovery workflows.<br=
 /><br />Key Takeaways:</td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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</tr>=20
<tr>=20
<td class=3D"textSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"text-=
align: left; vertical-align: top; font-size: 0px; line-height: 0px; height:=
 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
</table>
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quareOnWhite6ded5f90-b34d-4b1f-89a0-1af67af2824a" style=3D"border: none; bo=
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0; role: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width:=
 100%; background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspa=
cing=3D"0" role=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<td class=3D"globalModuleInner" style=3D"width: 560px; text-align: center; =
vertical-align: top;" width=3D"560" align=3D"center" valign=3D"top">=20
<table style=3D"width: 100%; border: none; border-collapse: collapse; borde=
r-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation; mso-tab=
le-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%" cellpadding=3D"0" ce=
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<tbody>=20
<tr>=20
<td class=3D" globalText bulletSquareColLeft " style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; text-align: left; color: #000000; vertica=
l-align: top; width: 25px; font-size: 12px; line-height: 22px;" width=3D"25=
" align=3D"left" valign=3D"top"> =E2=96=A0 </td>=20
<td class=3D"mktoText globalText bulletSquareColRight" id=3D"bulletSquareOn=
WhiteTextbce53629-adb9-427d-b3c5-ce585ed307f9" style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; font-size: 15px; line-height: 26px; text-=
align: left; color: #000000; vertical-align: top; width: 535px;" width=3D"5=
35" align=3D"left" valign=3D"top">Assess binding and specificity on live ce=
lls</td>=20
</tr>=20
</tbody>=20
</table> </td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
<tr>=20
<td class=3D"bulletSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"tex=
t-align: left; vertical-align: top; font-size: 0px; line-height: 0px; heigh=
t: 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
</table>
<table class=3D"mktoModule globalModule bullet bulletOnWhite" id=3D"bulletS=
quareOnWhite6bc08034-706b-450c-b5b3-374150711cb4" style=3D"border: none; bo=
rder-collapse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: =
0; role: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width:=
 100%; background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspa=
cing=3D"0" role=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"globalModuleInner" style=3D"width: 560px; text-align: center; =
vertical-align: top;" width=3D"560" align=3D"center" valign=3D"top">=20
<table style=3D"width: 100%; border: none; border-collapse: collapse; borde=
r-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation; mso-tab=
le-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%" cellpadding=3D"0" ce=
llspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td class=3D" globalText bulletSquareColLeft " style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; text-align: left; color: #000000; vertica=
l-align: top; width: 25px; font-size: 12px; line-height: 22px;" width=3D"25=
" align=3D"left" valign=3D"top"> =E2=96=A0 </td>=20
<td class=3D"mktoText globalText bulletSquareColRight" id=3D"bulletSquareOn=
WhiteText93e7c90e-efe0-4b27-a0d1-34e82450f1e7" style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; font-size: 15px; line-height: 26px; text-=
align: left; color: #000000; vertical-align: top; width: 535px;" width=3D"5=
35" align=3D"left" valign=3D"top">Identify mAbs that compete for epitope bi=
nding</td>=20
</tr>=20
</tbody>=20
</table> </td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
<tr>=20
<td class=3D"bulletSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"tex=
t-align: left; vertical-align: top; font-size: 0px; line-height: 0px; heigh=
t: 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
</table>
<table class=3D"mktoModule globalModule bullet bulletOnWhite" id=3D"bulletS=
quareOnWhitecf66c3f7-4bb0-46f4-889b-c372c2b58aef" style=3D"border: none; bo=
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0; role: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width:=
 100%; background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspa=
cing=3D"0" role=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"globalModuleInner" style=3D"width: 560px; text-align: center; =
vertical-align: top;" width=3D"560" align=3D"center" valign=3D"top">=20
<table style=3D"width: 100%; border: none; border-collapse: collapse; borde=
r-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation; mso-tab=
le-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%" cellpadding=3D"0" ce=
llspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td class=3D" globalText bulletSquareColLeft " style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; text-align: left; color: #000000; vertica=
l-align: top; width: 25px; font-size: 12px; line-height: 22px;" width=3D"25=
" align=3D"left" valign=3D"top"> =E2=96=A0 </td>=20
<td class=3D"mktoText globalText bulletSquareColRight" id=3D"bulletSquareOn=
WhiteText8da45e16-94b9-4382-ae9d-9f71bbb4eb22" style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; font-size: 15px; line-height: 26px; text-=
align: left; color: #000000; vertical-align: top; width: 535px;" width=3D"5=
35" align=3D"left" valign=3D"top">Rank antibodies based on glycosylation st=
atus</td>=20
</tr>=20
</tbody>=20
</table> </td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
<tr>=20
<td class=3D"bulletSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"tex=
t-align: left; vertical-align: top; font-size: 0px; line-height: 0px; heigh=
t: 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
</table>
<table class=3D"mktoModule globalModule bullet bulletOnWhite" id=3D"bulletS=
quareOnWhite86325adb-cf11-4615-9571-42a648c09599" style=3D"border: none; bo=
rder-collapse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: =
0; role: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width:=
 100%; background-color: #ffffff;" width=3D"100%" cellpadding=3D"0" cellspa=
cing=3D"0" role=3D"presentation" bgcolor=3D"#ffffff">=20
<tbody>=20
<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"globalModuleInner" style=3D"width: 560px; text-align: center; =
vertical-align: top;" width=3D"560" align=3D"center" valign=3D"top">=20
<table style=3D"width: 100%; border: none; border-collapse: collapse; borde=
r-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation; mso-tab=
le-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%" cellpadding=3D"0" ce=
llspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td class=3D" globalText bulletSquareColLeft " style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; text-align: left; color: #000000; vertica=
l-align: top; width: 25px; font-size: 12px; line-height: 22px;" width=3D"25=
" align=3D"left" valign=3D"top"> =E2=96=A0 </td>=20
<td class=3D"mktoText globalText bulletSquareColRight" id=3D"bulletSquareOn=
WhiteText64e09547-ef18-4908-8fc1-35255931d7a5" style=3D"font-family: Arial,=
 sans-serif; font-weight: normal; font-size: 15px; line-height: 26px; text-=
align: left; color: #000000; vertical-align: top; width: 535px;" width=3D"5=
35" align=3D"left" valign=3D"top">Analyze Fc receptor function in immune ce=
ll co-culture</td>=20
</tr>=20
</tbody>=20
</table> </td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
<tr>=20
<td class=3D"bulletSpacerBottom" colspan=3D"3" height=3D"10px" style=3D"tex=
t-align: left; vertical-align: top; font-size: 0px; line-height: 0px; heigh=
t: 10px;" align=3D"left" valign=3D"top"> &nbsp; </td>=20
</tr>=20
</tbody>=20
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