Da

✏️ Editing: 1683644242.M272598P1814477.aristoteles.x5servers.net,S=81857,W=83498:2,S

Envelope-to: rcruz@sapasequipment.com
Delivery-date: Tue, 09 May 2023 10:57:22 -0400
Date: Tue, 9 May 2023 08:56:35 -0600
From: Richard Casey <richard@thesequencingcenter.com>
Reply-To: richard@thesequencingcenter.com
To: rcruz@sapasequipment.com
Message-ID: <1683644065274.b97496f1-60da-4792-bbc0-618f2f7ad64f@bf02.hubspotfree.net>
Subject: HLA Typing Services
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Low cost HLA typing

dallas-divide-colorado-16-9 (https://cmgR004.na1.hubspotlinksfree.com/Ctc/G=
B+113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3l0fwV1-WJV7CgCZ=
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6L5yxK93W4txJpV8pF5TSV229KK1WVDsLW4gX5fn4D4RKFW6SXZpL3dV4rY3dHH1 )

HLA Newsletter

Discovering Recombination Events

in HLA Typing Results

At first, these results (https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+11=
3/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0Hy3l0gpV1-WJV7CgF83VFh=
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1RjxWx4f_QhMW2Q7rZB38mY4DW8Tt9vy7ssHZ4W1hbTR03vxZxc3nPG1 ) did not look rig=
ht.

In one of our client's HLA samples, there was a fairly large number of exon=
 and intron mismatches in HLA-DPA1.  But these only occurred downstream fro=
m exon 2.  Typically, we see no exon mismatches, or perhaps just a single m=
ismatch.  This is the nature of sequence alignments against the IMGT/HLA da=
tabase.

So we performed a deep dive into this dataset to see what was happening.  A=
fter a fair amount of sleuthing, we concluded that there was a novel allele=
 in this sample derived from a recombination event between DPA1*01:03:01:04=
 and DPA1*02:01:01:01.

The significance of this genetic recombination in disease states is current=
ly unknown.  However, it does show the power of Next Generation Sequencing =
when used for HLA Typing.

Our HLA Typing Services

Class I HLA Alleles

Whole gene coverage

- HLA-A
- HLA-B
- HLA-C
- HLA-G
- HLA-E (contact us)
- HLA-F (contact us)

Class II HLA Alleles

Extensive gene coverage

- HLA-DPA1
- HLA-DPB1
- HLA-DQA1
- HLA-DQB1
- HLA-DRB1
- HLA-DRB3
- HLA-DRB4
- HLA-DRB5

4-field resolution

Coverage includes all relevant exons, introns, 5'-UTR's, 3'-UTR's

Use 11-loci standard run or any combination of loci

DNA extraction

Next Generation Sequencing (Illumina, Nanopore)

Standardized, actionable HLA reports

Website (https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+113/cmgR004/VWPj6F=
8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3l0fwV1-WJV7CgPNwW3XGtv76WDLN7W8BHDz=
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The Sequencing Center, 1020 Luke Street, Suite D, Fort Collins, Colorado 80=
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                    <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc=
/GB+113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3lSbNV1-WJV7Cg=
WYYW2NSmp024CRXvW8wKlWd4bhQt7W3S_8qN2gspLdN8kCYJ4GbtGWW3p1Chh5DjCX0W2Cm4X83=
h8C_WW4z6XsL3d_c9wW71bdqj4lKxy_W4YLF4g5WG9WFW1XTS8B50KQpDW54nN7z9cpH17W4LKM=
6V1WtkJHW5WCHWV6_lVQGN8yfN5XG0y_wN20kKPzCKDpxW81GCjc8Pmv76N7zTkFyr8JPwW84cr=
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=3D"_blank" style=3D"color:#00a4bd; mso-line-height-rule:exactly" data-hs-l=
ink-id=3D"0">
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                <img alt=3D"dallas-divide-colorado-16-9" src=3D"https://hs-=
3035016.f.hubspotfree.net/hub/3035016/hubfs/dallas-divide-colorado-16-9.jpg=
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" style=3D"outline:none; text-decoration:none; -ms-interpolation-mode:bicub=
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ue&amp;name=3Ddallas-divide-colorado-16-9.jpg 560w, https://hs-3035016.f.hu=
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6-9.jpg 1120w" sizes=3D"(max-width: 560px) 100vw, 560px">
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                    </a>
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            </td>
        </tr>
    </tbody>
</table></div>
</div>


    
    </div>
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  </div>
  <div id=3D"section-2" class=3D"hse-section" style=3D"padding-left:10px; p=
adding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
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<div id=3D"column-2-0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module-2-0-0" class=3D"hs_cos_=
wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: i=
nherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-type=
=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_module-2-=
0-0_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_ric=
h_text" style=3D"color: inherit; font-size: inherit; line-height: inherit;"=
 data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_text"><h1 sty=
le=3D"margin:0; mso-line-height-rule:exactly; line-height:175%; font-size:2=
4px; text-align:center" align=3D"center">HLA Newsletter</h1></div></div></t=
d></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826157720115" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module">

<table role=3D"presentation" width=3D"100%" align=3D"center" border=3D"0" s=
tyle=3D"position:relative; top:-1px; min-width:20px; width:100%; max-width:=
100%; border-spacing:0; mso-table-lspace:0pt; mso-table-rspace:0pt; border-=
collapse:collapse; font-size:1px">
  <tbody><tr>
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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    <td width=3D"50%" valign=3D"middle" style=3D"border-collapse:collapse; =
mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-size:15px=
; color:#23496d; word-break:break-word; line-height:0; border:transparent; =
border-bottom:1px solid #99acc2; mso-border-bottom-alt:2pt solid #99acc2; b=
order-bottom-width:1px"></td>
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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  </tr>
</tbody></table>
</div></td>=
</tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16831228957192" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_mo=
dule_16831228957192_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_=
wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-h=
eight: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"ri=
ch_text"><h2 style=3D"margin:0; mso-line-height-rule:exactly; font-size:20p=
x; line-height:175%; text-align:center" align=3D"center">Discovering Recomb=
ination Events</h2>
<h2 style=3D"margin:0; mso-line-height-rule:exactly; font-size:20px; line-h=
eight:175%; text-align:center" align=3D"center">in HLA Typing Results</h2>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">At first, <a hr=
ef=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+113/cmgR004/VWPj6F8Rf=
_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0Hy3lScGV1-WJV7CgH_7Vg_hK06gsXvbW63XnlL1SS=
R3cVDkQV72vgm8pW3bzX3d1SlGjjW5zMk9W11qc5RW7Y8wQY9cPc2tW9lqJFs4xVff6W61XRbY2=
Nd4dHN172x7HXjQDvW1GcdD47b19zkW1JcLXp29Lv6MW7wl7cT6pPRTdW75FBb155hLMrW6FMzZ=
G55vS7zW3rGd1P87WKKRW2pNc-87TBlBFVJNB_399GzKnW29tzrx26qHMbW4MWQHb5L5tTPW7dg=
NXW6n7pdLN3LvLwlQl9qQN8SRHszjPshGW6-cCRC9jwtvKW41zt1Z1WThFpW1g6S7P6S_KRkW8b=
YtbN1W_bksW7lkhvw1_Ltt9W41w9cG86bClD39wS1" rel=3D"noopener" style=3D"mso-li=
ne-height-rule:exactly; color:#00a4bd" data-hs-link-id=3D"0" target=3D"_bla=
nk">these results</a> did not look right.</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">In one of our c=
lient's HLA samples, there was a fairly large number of exon and intron mis=
matches in HLA-DPA1.&nbsp; But these only occurred downstream from exon 2.&=
nbsp; Typically, we see no exon mismatches, or perhaps just a single mismat=
ch.&nbsp; This is the nature of sequence alignments against the IMGT/HLA da=
tabase.</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">So we performed=
 a deep dive into this dataset to see what was happening.&nbsp; After a fai=
r amount of sleuthing, we concluded that there was a novel allele in this s=
ample derived from a recombination event between DPA1*01:03:01:04 and DPA1*=
02:01:01:01.&nbsp;&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">The significanc=
e of this genetic recombination in disease states is currently unknown.&nbs=
p; However, it does show the power of Next Generation Sequencing when used =
for HLA Typing.&nbsp;&nbsp;</p></div></div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16831228771001" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module">

<table role=3D"presentation" width=3D"100%" align=3D"center" border=3D"0" s=
tyle=3D"position:relative; top:-1px; min-width:20px; width:100%; max-width:=
100%; border-spacing:0; mso-table-lspace:0pt; mso-table-rspace:0pt; border-=
collapse:collapse; font-size:1px">
  <tbody><tr>
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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    <td width=3D"50%" valign=3D"middle" style=3D"border-collapse:collapse; =
mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-size:15px=
; color:#23496d; word-break:break-word; line-height:0; border:transparent; =
border-bottom:1px solid #99acc2; mso-border-bottom-alt:2pt solid #99acc2; b=
order-bottom-width:1px"></td>
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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  </tr>
</tbody></table>
</div></td>=
</tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16831230001263" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_mo=
dule_16831230001263_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_=
wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-h=
eight: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"ri=
ch_text"><h2 style=3D"margin:0; mso-line-height-rule:exactly; line-height:1=
75%; font-size:22px; text-align:center" align=3D"center">Our HLA Typing Ser=
vices</h2></div></div></td></tr></tbody></table>
</div>


    
    </div>
  =20
  </div>
  <div id=3D"section_1682615069302" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682615069302_0" class=3D"hse-column hse-size-6">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826150782651" class=
=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapp=
er_module_16826150782651_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs=
_cos_wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; l=
ine-height: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=
=3D"rich_text"><h2 style=3D"margin:0; mso-line-height-rule:exactly; font-si=
ze:20px; line-height:175%">Class I HLA Alleles</h2>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">Whole gene cove=
rage</p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%">
<li style=3D"mso-line-height-rule:exactly">HLA-A</li>
<li style=3D"mso-line-height-rule:exactly">HLA-B</li>
<li style=3D"mso-line-height-rule:exactly">HLA-C</li>
<li style=3D"mso-line-height-rule:exactly">HLA-G</li>
<li style=3D"mso-line-height-rule:exactly">HLA-E (contact us)</li>
<li style=3D"mso-line-height-rule:exactly">HLA-F (contact us)</li>
</ul></div></div></td></tr></tbody></table>
</div>




<div id=3D"column_1682615069302_1" class=3D"hse-column hse-size-6">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826153209922" class=
=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapp=
er_module_16826153209922_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs=
_cos_wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; l=
ine-height: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=
=3D"rich_text"><h2 style=3D"margin:0; mso-line-height-rule:exactly; font-si=
ze:20px; line-height:175%">Class II HLA Alleles</h2>
<p style=3D"mso-line-height-rule:exactly; line-height:175%">Extensive gene =
coverage</p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%">
<li style=3D"mso-line-height-rule:exactly">HLA-DPA1</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DPB1</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DQA1</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DQB1</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DRB1</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DRB3</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DRB4</li>
<li style=3D"mso-line-height-rule:exactly">HLA-DRB5</li>
</ul></div></div></td></tr></tbody></table>
</div>


    
    </div>
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  </div>
  <div id=3D"section_1682616412962" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682616412962_0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826176626849" class=
=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module">

<table role=3D"presentation" width=3D"100%" align=3D"center" border=3D"0" s=
tyle=3D"position:relative; top:-1px; min-width:20px; width:100%; max-width:=
100%; border-spacing:0; mso-table-lspace:0pt; mso-table-rspace:0pt; border-=
collapse:collapse; font-size:1px">
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
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    <td width=3D"50%" valign=3D"middle" style=3D"border-collapse:collapse; =
mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-size:15px=
; color:#23496d; word-break:break-word; line-height:0; border:transparent; =
border-bottom:1px solid #99acc2; mso-border-bottom-alt:2pt solid #99acc2; b=
order-bottom-width:1px"></td>
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    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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  </tr>
</tbody></table>
</div></td>=
</tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826164213187" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_mo=
dule_16826164213187_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_=
wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-h=
eight: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"ri=
ch_text"><p style=3D"mso-line-height-rule:exactly; line-height:175%; text-a=
lign:center" align=3D"center">4-field resolution</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%; text-align:cent=
er" align=3D"center">Coverage includes all relevant exons, introns, 5'-UTR'=
s, 3'-UTR's</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%; text-align:cent=
er" align=3D"center">Use 11-loci standard run or any combination of loci&nb=
sp;</p></div></div></td></tr></tbody></table>
</div>


    
    </div>
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  </div>
  <div id=3D"section_1682615510332" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
   =20
   =20
    
      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682615510332_0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826157571214" class=
=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module">

<table role=3D"presentation" width=3D"100%" align=3D"center" border=3D"0" s=
tyle=3D"position:relative; top:-1px; min-width:20px; width:100%; max-width:=
100%; border-spacing:0; mso-table-lspace:0pt; mso-table-rspace:0pt; border-=
collapse:collapse; font-size:1px">
  <tbody><tr>
   =20
   =20
    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
   =20
   =20
    <td width=3D"50%" valign=3D"middle" style=3D"border-collapse:collapse; =
mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-size:15px=
; color:#23496d; word-break:break-word; line-height:0; border:transparent; =
border-bottom:1px solid #99acc2; mso-border-bottom-alt:2pt solid #99acc2; b=
order-bottom-width:1px"></td>
   =20
   =20
    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
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  </tr>
</tbody></table>
</div></td>=
</tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; pa=
dding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826155227173" class=3D"=
hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"c=
olor: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-gener=
al-type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_mo=
dule_16826155227173_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_=
wrapper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-h=
eight: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"ri=
ch_text"><p style=3D"mso-line-height-rule:exactly; line-height:175%; text-a=
lign:center" align=3D"center">DNA extraction&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%; text-align:cent=
er" align=3D"center">Next Generation Sequencing (Illumina, Nanopore)</p>
<p style=3D"mso-line-height-rule:exactly; line-height:175%; text-align:cent=
er" align=3D"center">Standardized, actionable HLA reports</p></div></div></=
td></tr></tbody></table>
</div>


    
    </div>
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  </div>
  <div id=3D"section_1682616813147" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682616813147_0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module_16826159952386" class=
=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module">

<table role=3D"presentation" width=3D"100%" align=3D"center" border=3D"0" s=
tyle=3D"position:relative; top:-1px; min-width:20px; width:100%; max-width:=
100%; border-spacing:0; mso-table-lspace:0pt; mso-table-rspace:0pt; border-=
collapse:collapse; font-size:1px">
  <tbody><tr>
   =20
   =20
    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
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   =20
   =20
    <td width=3D"50%" valign=3D"middle" style=3D"border-collapse:collapse; =
mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-size:15px=
; color:#23496d; word-break:break-word; line-height:0; border:transparent; =
border-bottom:1px solid #99acc2; mso-border-bottom-alt:2pt solid #99acc2; b=
order-bottom-width:1px"></td>
   =20
   =20
    <td width=3D"auto" style=3D"border-collapse:collapse; mso-line-height-r=
ule:exactly; font-family:Arial, sans-serif; font-size:15px; color:#23496d; =
word-break:break-word; line-height:0"></t=
d>
   =20
  </tr>
</tbody></table>
</div></td>=
</tr></tbody></table>
</div>


    
    </div>
  =20
  </div>
  <div id=3D"section_1682616818364" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
   =20
   =20
    
      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682616818364_0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module-5-0-0" class=3D"hs_cos_=
wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: i=
nherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-type=
=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_module-5-=
0-0_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_ric=
h_text" style=3D"color: inherit; font-size: inherit; line-height: inherit;"=
 data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_text"><p styl=
e=3D"mso-line-height-rule:exactly; line-height:175%; text-align:center; mar=
gin-bottom:10px" align=3D"center">Contact us for a quote or free consultati=
on.</p></div></div></td></tr></tbody></table>
</div>


    
    </div>
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  </div>
  <div id=3D"section-5" class=3D"hse-section" style=3D"padding-left:10px; p=
adding-right:10px">
   =20
   =20
    
      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff; padding-bottom:20px; =
padding-top:20px" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column-5-0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
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=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=
=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
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<table role=3D"presentation" align=3D"center" style=3D"border-spacing:0 !im=
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pt; width:auto; text-align:center" class=3D"hs_cos_wrapper_type_social_modu=
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  <tbody>
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rspace:0pt; width:auto; text-align:center" class=3D"hs_cos_wrapper_type_soc=
ial_module_single" width=3D"auto">
          <tbody>
            <tr align=3D"center">
             =20
              <td class=3D"display_block_on_small_screens" style=3D"border-=
collapse:collapse; mso-line-height-rule:exactly; font-family:Arial, sans-se=
rif; font-size:15px; color:#23496d; word-break:break-word; padding:8px 5px;=
 line-height:1; vertical-align:middle" valign=3D"middle">
                <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+=
113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3lSbNV1-WJV7CgQmWW=
7mmxbv2dTDYbN4Z-z-Vj6JDGW1qDpt32wdZwVN4c_K2WstrRtW48Vxx85NGrC_W7_yHFk5rmMRj=
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tc-N2twQnS7gb-3W53052G8mpbrsVj2Zv28ZlZHdW708DQk19Rn9_VVsGs299xYfHN1XYNMCQQ4=
YwW7xJB1Z8Z9F8LW2VBTHv2Zv1m_W3wpkTl6W3wcnW7TwX4J4G-nzL39-B1" style=3D"color=
:#00a4bd; mso-line-height-rule:exactly; text-decoration:none !important" da=
ta-hs-link-id=3D"0" target=3D"_blank">
                 =20
                 =20
                  <img src=3D"https://hs-3035016.f.hubspotfree.net/hs/hssta=
tic/TemplateAssets/static-1.114/img/hs_default_template_images/modules/Foll=
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                </a>
              </td>
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            </tr>
          </tbody>
        </table>
      </td>
     =20
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     =20
      <td class=3D"social-network-cell" style=3D"border-collapse:collapse; =
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          <tbody>
            <tr align=3D"center">
             =20
              <td class=3D"display_block_on_small_screens" style=3D"border-=
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rif; font-size:15px; color:#23496d; word-break:break-word; padding:8px 5px;=
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                <a href=3D"mailto:info@thesequencingcenter.com" style=3D"co=
lor:#00a4bd; mso-line-height-rule:exactly; text-decoration:none !important"=
 data-hs-link-id=3D"0" target=3D"_blank">
                 =20
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lign=3D"middle" width=3D"auto">
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                </a>
              </td>
             =20
             =20
            </tr>
          </tbody>
        </table>
      </td>
     =20
     =20
       =20
     =20
      <td class=3D"social-network-cell" style=3D"border-collapse:collapse; =
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        <table role=3D"presentation" align=3D"center" style=3D"border-spaci=
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ial_module_single" width=3D"auto">
          <tbody>
            <tr align=3D"center">
             =20
              <td class=3D"display_block_on_small_screens" style=3D"border-=
collapse:collapse; mso-line-height-rule:exactly; font-family:Arial, sans-se=
rif; font-size:15px; color:#23496d; word-break:break-word; padding:8px 5px;=
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                <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+=
113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GZ3lSc3V1-WJV7CgX2TW=
50TKbC1lVYk6W4cBfxc4_wFyMV7frfW6CF04xW40Nvng84YVk_W87fWp_3QvhwXW40R9z61gcF2=
FVgTHMX1rvP7dW2t-Wxp2TtM2bW76ynw34xRjnYW2jLBZb9jXgC6W3CgXFp3fpbzXW718jkl5gq=
3qSW5nKRjr17tRbGW6wphZG3X1P02W6zt9lR2hlSz7W36jz-Z1zltj4W1qDkzL7hkjDLW8G-jcY=
8JN8RSW4qB1tG8LMGbqW19Z7bV5CPs5MW9h213M8hnFmvW2clKpw28pvBCW5g-QLS71KrdDW2T-=
F4W3KmQy2368b1" style=3D"color:#00a4bd; mso-line-height-rule:exactly; text-=
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                 =20
                 =20
                  <img src=3D"https://hs-3035016.f.hubspotfree.net/hs/hssta=
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                </a>
              </td>
             =20
             =20
            </tr>
          </tbody>
        </table>
      </td>
     =20
     =20
       =20
     =20
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             =20
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113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3lSbNV1-WJV7CgBLDW=
9bBZ0H54JQqzW5vJvmB6XV3PMW1x5m6B7jc7XsW2BDn1M2FW1b1VLMTWM4RscJvN5SfNk1nDtJq=
W17hjl51qGXP3VB9Cmh5sGLxMVBvL1H2GTfSbVpX_y93_y4MdW7mlZDf5_lz9GVrZ0vs5Rb1RGW=
8_zlMM7T2LyFW1VhtBs6KW0k0Vz8XCN7Z5HCbW1JQ4gK6jjVwMW4FvkQw7F2-3dVCjSLV510PLX=
W51ZB3v6D-GF_W5dF4tV2L7gThW4jwddQ4f0kQXW40vVf08v_BD_3dV91" style=3D"color:#=
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-hs-link-id=3D"0" target=3D"_blank">
                 =20
                 =20
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e" valign=3D"middle" width=3D"auto">
                 =20
                </a>
              </td>
             =20
             =20
            </tr>
          </tbody>
        </table>
      </td>
     =20
    </tr>
  </tbody>
</table>
</div></td></tr></tbody></table>
</div>


    
    </div>
  =20
  </div>
  <div id=3D"section_1682616835729" class=3D"hse-section" style=3D"padding-=
left:10px; padding-right:10px">
   =20
   =20
    
      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; background-color:#ffffff" bgcolor=3D"#ffffff">
    
   =20
    
    

<div id=3D"column_1682616835729_0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
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y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
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=3D"color: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-=
general-type=3D"widget" data-hs-cos-type=3D"module">
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  <table width=3D"100%" align=3D"center" border=3D"0" cellpadding=3D"0" cel=
lspacing=3D"0" role=3D"presentation" style=3D"border-spacing:0 !important; =
mso-table-lspace:0pt; mso-table-rspace:0pt; border-collapse:separate!import=
ant">
    <tbody><tr>
        
    
      <td align=3D"center" valign=3D"middle" bgcolor=3D"#FF9902" role=3D"pr=
esentation" style=3D"border-collapse:collapse; mso-line-height-rule:exactly=
; font-family:Arial, sans-serif; font-size:15px; color:#23496d; word-break:=
break-word; border-radius:8px; cursor:auto; background-color:#FF9902">
   
        <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc/GB+113/cmgR=
004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0GG3lSbNV1-WJV7CgCp9N2jfrBDvf=
Jp-W8V2bH04dPp_hMVYHzzgyrGfW8-Kqx77QlvgwW3P0GC91CCHvvW3s_8sl50PQ0CW44_v0Z8d=
Vj_ZW90mybp1j7fDdW27YQm-4VXWh9W1vjlwN2R_F1GW4Y7Xv073G68MW8X2Sjq2JC7XZW6-gFq=
F7FwfNpN8b6N8ZYhnt8W3bzDt87hsdlWW5J8c7F6VXw-QN2Yk3pjLDCZCW3Vdv1w7Gh6CxVZ7nr=
W7YYj5bVQWMtN6983WTW4GJdJR73g32LW3drYTN2R42Vc3cNq1" target=3D"_blank" style=
=3D"color:#00a4bd; mso-line-height-rule:exactly; font-size:16px; font-famil=
y:Arial, sans-serif; Margin:0; text-transform:none; text-decoration:none; p=
adding:12px 18px; display:block" data-hs-link-id=3D"0">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">Get a Quote</strong>
        </a>
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    </div>
  =20
  </div>
  <div id=3D"section-6" class=3D"hse-section" style=3D"padding-left:10px; p=
adding-right:10px">
   =20
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      <div class=3D"hse-column-container" style=3D"min-width:280px; max-wid=
th:600px; width:100%; Margin-left:auto; Margin-right:auto; border-collapse:=
collapse; border-spacing:0; padding-top:30px">
    
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<div id=3D"column-6-0" class=3D"hse-column hse-size-12">
  <table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D=
"100%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
y:Arial, sans-serif; font-size:15px; color:#23496d; word-break:break-word; =
padding:10px 20px"><div id=3D"hs_cos_wrapper_module-6-0-0" class=3D"hs_cos_=
wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: i=
nherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-type=
=3D"widget" data-hs-cos-type=3D"module">
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<table role=3D"presentation" class=3D"hse-footer hse-secondary" width=3D"10=
0%" cellpadding=3D"0" cellspacing=3D"0" style=3D"border-spacing:0 !importan=
t; border-collapse:collapse; mso-table-lspace:0pt; mso-table-rspace:0pt; fo=
nt-family:Arial, sans-serif; font-size:12px; line-height:135%; color:#23496=
d; margin-bottom:0; padding:0">
    <tbody>
        <tr>
            <td align=3D"center" valign=3D"top" style=3D"border-collapse:co=
llapse; mso-line-height-rule:exactly; font-family:Arial, sans-serif; font-s=
ize:15px; color:#23496d; word-break:break-word; text-align:center; margin-b=
ottom:0; line-height:135%; padding:10px 20px">
               =20
                <p style=3D"mso-line-height-rule:exactly; font-family:Arial=
, sans-serif; font-size:12px; font-weight:normal; text-decoration:none; fon=
t-style:normal; color:#23496d; direction:lrt" dir=3D"lrt">
                  The Sequencing Center, 1020 Luke Street, Suite D, Fort Co=
llins, Colorado 80524, USA, 877-425-2235
                </p>
                <p style=3D"mso-line-height-rule:exactly">
                 =20
                  <a data-unsubscribe=3D"true"  href=3D"https://hs-3035016.=
s.hubspotfree.net/email-unsubscribe/email?product=3DemailStarter&amp;checkS=
ubscriptions=3Dall&amp;d=3DVngbxM5S7TZ0VWdKXH4hPQt1W3zfNyt3K8pPtW45RL1d4fdH=
ZSW3_fWvc8629K7VmWFft6kbbfdN6vqk-V25r-3W8xNk7M62_TBLVXhvCK4_kGxqMXk_7QbvYnw=
W7xdMfK36G_1nW8knrbW7M3SMX3lPM2&amp;v=3D2&amp;email=3Drcruz%40sapasequipmen=
t.com&amp;utm_source=3Dhs_email&amp;utm_medium=3Demail&amp;utm_content=3D25=
7576525&amp;_hsenc=3Dp2ANqtz-95q_q9NonKqn_ckF5FAqTIuat4kR6MESb_VgEH7-c5WjRP=
Ob0TXWUengxFzsEBpUnbPMXiOJGXD-32AgxmhD5QIR7-eA&amp;_hsmi=3D257576525" style=
=3D"mso-line-height-rule:exactly; font-family:Helvetica,Arial,sans-serif; f=
ont-size:12px; color:#00a4bd; font-weight:normal; text-decoration:underline=
; font-style:normal" data-hs-link-id=3D"0" target=3D"_blank">Unsubscribe</a=
>
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                  <a data-unsubscribe=3D"true"  href=3D"https://hs-3035016.=
s.hubspotfree.net/email-unsubscribe/email?product=3DemailStarter&amp;d=3DVn=
gbxM5S7TZ0VWdKXH4hPQt1W3zfNyt3K8pPtW45RL1d4fdHZSW3_fWvc8629K7VmWFft6kbbfdN6=
vqk-V25r-3W8xNk7M62_TBLVXhvCK4_kGxqMXk_7QbvYnwW7xdMfK36G_1nW8knrbW7M3SMX3lP=
M2&amp;v=3D2&amp;email=3Drcruz%40sapasequipment.com&amp;utm_source=3Dhs_ema=
il&amp;utm_medium=3Demail&amp;utm_content=3D257576525&amp;_hsenc=3Dp2ANqtz-=
95q_q9NonKqn_ckF5FAqTIuat4kR6MESb_VgEH7-c5WjRPOb0TXWUengxFzsEBpUnbPMXiOJGXD=
-32AgxmhD5QIR7-eA&amp;_hsmi=3D257576525" style=3D"mso-line-height-rule:exac=
tly; font-family:Helvetica,Arial,sans-serif; font-size:12px; color:#00a4bd;=
 font-weight:normal; text-decoration:underline; font-style:normal" data-hs-=
link-id=3D"0" target=3D"_blank">Manage preferences</a>
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</div>


    
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  =20
  </div>
<div id=3D"section_hubspot_brand" class=3D"hse-section branding-design-1 br=
anding-box-design " style=3D"padding-left:10px; padding-right:10px">
    
      <div class=3D"hse-column-container" width=3D"100%" style=3D"min-width=
:280px; max-width:600px; width:100%; Margin-left:auto; Margin-right:auto; b=
order-collapse:collapse; border-spacing:0; padding-bottom:20px; padding-top=
:20px">
    
    
<div id=3D"column_hubspot_brand" class=3D"hse-column hse-size-12">
  <div id=3D"hs_cos_wrapper_module_hubspot_brand">
  <table role=3D"presentation" width=3D"100%" cellpadding=3D"0" cellspacing=
=3D"0" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-=
table-lspace:0pt; mso-table-rspace:0pt">
      <tbody>
      <tr><td class=3D"moduleAlignmentCell" align=3D"center" style=3D"borde=
r-collapse:collapse; mso-line-height-rule:exactly; font-family:Arial, sans-=
serif; font-size:15px; color:#23496d; word-break:break-word">
        <img src=3D"https://api-na1.hubspot.com/viral-links/v1/tracking?isE=
xternalLink=3Dtrue&amp;viralLinkType=3Dmarketing-email&amp;deviceId=3D2fb57=
992-bf69-4a70-ab2e-b1118452becb&amp;hubId=3D3035016" style=3D"outline:none;=
 text-decoration:none; -ms-interpolation-mode:bicubic; display:none">
        <table role=3D"presentation" width=3D"200" cellpadding=3D"0" cellsp=
acing=3D"0" style=3D"border-spacing:0 !important; border-collapse:collapse;=
 mso-table-lspace:0pt; mso-table-rspace:0pt; width:200px; background-color:=
#303E4F" bgcolor=3D"#303E4F">
            <tbody>
                <tr>
                  <td id=3D"hubspot-branding-text" style=3D"border-collapse=
:collapse; mso-line-height-rule:exactly; font-family:Arial, sans-serif; fon=
t-size:15px; color:#23496d; word-break:break-word; text-align:center; paddi=
ng-top:20px" align=3D"center">
                  <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc/G=
B+113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0G37mf_BV5X_Kf7CgWW=
5N7cJGYW9RGP1W454w5367sngRW2df-pQ8FLj5_W3jqsZK2kc9VxVqQkcJ1zddmcW6gQ18g34h4=
4tVF167W8XwD32W1Xf1Qk6zhYHdW6K_G9k7lcxcxW62lmph6K9w22N1FSbL7Fj2SnW7t9ywC1Ns=
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5rSzV_W19KTJr5ZP22YW4-M95s720pf0W2wYX0q5WgZPYW5qznh16d2FBQW1JRpy-7kJh5jW60v=
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nBkyh500wg6W71N5Mp2lqp5_W5YFQmf8d61LrW2_tQFB728K98V2bYL68dbxgbW48KmDk5wFkGy=
W958Bw-1RSgkHW2hQVXs8bdYLJVW476D7Kphxc31P-1" rel=3D"nofollow noopener" data=
-insert=3D"true" data-hs-link-id=3D"0" data-hubspot-branding=3D"true" targe=
t=3D"_blank" style=3D"mso-line-height-rule:exactly; text-decoration:none; c=
olor:#ffffff; font-size:13px; font-family:Arial,sans-serif">Send free email=
 today</a>
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                </tr>
                <tr>
                    <td align=3D"center" valign=3D"top" style=3D"border-col=
lapse:collapse; mso-line-height-rule:exactly; font-family:Arial, sans-serif=
; font-size:15px; color:#23496d; word-break:break-word; padding-bottom:20px=
">
                    <a href=3D"https://cmgR004.na1.hubspotlinksfree.com/Ctc=
/GB+113/cmgR004/VWPj6F8Rf_2VW5mwX7Q5dwW4qW2VV8NG4-lL4DN97h0G37mf_BV5X_Kf7Cg=
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target=3D"_blank" style=3D"color:#00a4bd; mso-line-height-rule:exactly; tex=
t-decoration:none">
                        <img alt=3D"HubSpot" src=3D"https://hs-3035016.f.hu=
bspotfree.net/hs/hsstatic/TemplateAssets/static-1.31/img/hs_default_templat=
e_images/email_dnd_template_images/hubspot-logo-white.png" style=3D"outline=
:none; text-decoration:none; -ms-interpolation-mode:bicubic; border:none; m=
ax-width:80px" width=3D"80" height=3D"31" align=3D"middle">
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<img src=3D"https://cmgR004.na1.hubspotlinksfree.com/Cto/GB+113/cmgR004/VWP=
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;padding-top:0!important;padding-bottom:0!important;padding-right:0!importa=
nt;padding-left:0!important"></body></html>
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