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✏️ Editing: 1687527199.M285794P2942425.aristoteles.x5servers.net,S=53512,W=54880:2,

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Delivery-date: Fri, 23 Jun 2023 09:33:19 -0400
Date: Fri, 23 Jun 2023 08:32:31 -0500 (CDT)
From: Sartorius <news@sartorius.com>
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Message-ID: <1213462099.10157627.1687527151472@nld1mktmail-batch1e.marketo.org>
Subject: Streamline ADCs Characterization
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Characterizing Trastuzumab ADCs with BLI and Advanced Flow Cytometry
New Application Note
Antibody-drug conjugates (ADCs) are among the fastest growing class of biol=
ogics and are developed as a combination of monoclonal antibodies (mAbs) an=
d highly potent cytotoxic molecules (drugs). The antibody, in addition to i=
ts own biological specificity through its paratope, has the function of a t=
argeted transporting tool of the drug to the targeted cell. The conjugated =
molecules or particles typically have cytotoxic activity and thus increase =
the efficiency of the antibody by an additional mechanism, leading to incre=
ased death of cells carrying the target antigen.=20

Conjugation of these drugs to antibodies, however, increases structural co=
mplexity which triggers the need for improved characterization methods util=
izing technologies that can add biophysical and functional information.=20

This application note <https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22=
Ha21Hi0fNIb7tAMQp6YqXMo52RG5tHuaYfFmHDnnJarHntrKw2B0q4e6nbIpORUildZ4=3D> de=
monstrates the use of Octet=C2=AE bio-layer=20
interferometry (BLI) platform in combination with the iQue=C2=AE Advanced F=
low Cytometry Platform to characterize and compare the drug trastuzumab to =
the trastuzumab-containing reference medicinal products (RMPs) Kadcyla=C2=
=AE and Enhertu=C2=AE.

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<table class=3D"mktoModule globalModule headline headlineOnWhite" id=3D"h1O=
nWhite4f632d27-805e-4190-bc3b-ec421a8ea5ec" style=3D"border: none; border-c=
ollapse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; rol=
e: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 100%;=
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; color: #000000; vertical-align: top; font-size: 30px; line-height: 36px;"=
 width=3D"560" align=3D"left" valign=3D"top">Characterizing Trastuzumab ADC=
s with BLI and Advanced Flow Cytometry</td>=20
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gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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nWhiteba93a528-45e8-485e-8141-4a21afeac94e" style=3D"border: none; border-c=
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; color: #000000; vertical-align: top; font-size: 22px; line-height: 26px;"=
 width=3D"560" align=3D"left" valign=3D"top">New Application Note</td>=20
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gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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ext-align: left; vertical-align: top; font-size: 0px; line-height: 0px; hei=
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</tr>=20
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<table class=3D"mktoModule globalModule text textOnWhite" id=3D"textOnWhite=
31408295-9245-4f11-9270-ae1a9279ca1c" style=3D"border: none; border-collaps=
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sentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 100%; backg=
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<tr>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<td class=3D"mktoText globalModuleInner globalText textText " id=3D"textOnW=
hiteText522425a5-381d-44cf-b42a-465148139529" style=3D"width: 560px; font-f=
amily: Arial, sans-serif; font-weight: normal; font-size: 15px; line-height=
: 26px; text-align: left; color: #000000; vertical-align: top;" width=3D"56=
0" align=3D"left" valign=3D"top">Antibody-drug conjugates (ADCs) are among =
the fastest growing class of biologics and are developed as a combination o=
f monoclonal antibodies (mAbs) and highly potent cytotoxic molecules (drugs=
). The antibody, in addition to its own biological specificity through its =
paratope, has the function of a targeted transporting tool of the drug to t=
he targeted cell. The conjugated molecules or particles typically have cyto=
toxic activity and thus increase the efficiency of the antibody by an addit=
ional mechanism, leading to increased death of cells carrying the target an=
tigen. <br /><br /> Conjugation of these drugs to antibodies, however, incr=
eases structural complexity which triggers the need for improved characteri=
zation methods utilizing technologies that can add biophysical and function=
al information. <br /><br /> This <a href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha64w9AiYNkP5pcwM_peVVE=
fxSRhqnp-XtIUqCrZ51fMZxFyjrWOYCxnicAqHS9a5xv8=3D" target=3D"_blank" id=3D""
>application note</a> demonstrates the use of Octet<sup>=C2=AE</sup> bio-la=
yer interferometry (BLI) platform in combination with the iQue<sup>=C2=AE</=
sup> Advanced Flow Cytometry Platform to characterize and compare the drug =
trastuzumab to the trastuzumab-containing reference medicinal products (RMP=
s) Kadcyla<sup>=C2=AE</sup> and Enhertu<sup>=C2=AE</sup>. </td>=20
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gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
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<tbody>=20
<tr>=20
<th class=3D"footerSocialColLeft" style=3D"text-align: left; font-weight: n=
ormal; width: 260px; font-size: 12px; line-height: 16px; vertical-align: mi=
ddle;" width=3D"260" align=3D"left" valign=3D"middle">=20
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r-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation; mso-tab=
le-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%" cellpadding=3D"0" ce=
llspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td style=3D"text-align: left; vertical-align: top;" align=3D"left" valign=
=3D"top">=20
<table class=3D"btnOutline" style=3D"width: 100%; border: none; border-coll=
apse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; role: =
presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt;" width=3D"100%"=
 cellpadding=3D"0" cellspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td style=3D"text-align: left; vertical-align: top;" align=3D"left" valign=
=3D"top">=20
<table class=3D"btnOutlineWrapper" style=3D"border: none; border-collapse: =
collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presen=
tation; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 100%; text-ali=
gn: left;" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" role=3D"prese=
ntation" align=3D"left">=20
<tbody>=20
<tr>=20
<td class=3D"btnOutlineInner" style=3D"text-align: left; vertical-align: to=
p;" align=3D"left" valign=3D"top"><a class=3D"btnOutlineLink btnOutlineLink=
ColorWhite btnOutlineLinkSpecialFooter" href=3D
"https://click.sartorius.com/dc/5HL5y5hF-ppxojJP01Mr_MiD_DCPohBzE6CE9g_dn-3=
CAlQ7LBQyn0pzADJATX9yWafCz4KYcjbOZ6rbuSFjt37CpfZMGr8yDx7xrm_k9SW6fZyEzLDFcv=
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tyle=3D"display: inline-block; padding: 10px 25px;=20
font-family: Arial,                                                        =
        sans-serif; font-size: 16px; font-weight: normal; line-height: 18px=
; text-decoration: none; text-align: center; color: #ffffff; border: 1px so=
lid                                                                #ffffff;=
"
> Contact Us </a></td>=20
</tr>=20
</tbody>=20
</table> </td>=20
</tr>=20
</tbody>=20
</table> </td>=20
</tr>=20
<tr>=20
<td class=3D"footerSocialSpacerBottom" style=3D"text-align: left; vertical-=
align: top; height: 20px; font-size: 0px; line-height: 0px;" height=3D"20" =
align=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
</tbody>=20
</table> </th>=20
<th class=3D"footerSocialColSpacerHor" style=3D"text-align: left; vertical-=
align: top; font-weight: normal; width: 20px; font-size: 0px; line-height: =
0px;" width=3D"20" align=3D"left" valign=3D"top">&nbsp;</th>=20
<th class=3D"footerSocialColRight" style=3D"font-weight: normal; width: 280=
px; text-align: right; vertical-align: middle;" width=3D"280" align=3D"righ=
t" valign=3D"middle">=20
<table class=3D"footerSocialList" style=3D"width: 100%; border: none; borde=
r-collapse: collapse; border-spacing: 0px; cellpadding: 0; cellspacing: 0; =
role: presentation; mso-table-lspace: 0pt; mso-table-rspace: 0pt;" width=3D=
"100%" cellpadding=3D"0" cellspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td class=3D"footerSocialListItem" style=3D"text-align: left; vertical-alig=
n: top; width: 40px;" width=3D"40" align=3D"left" valign=3D"top"><a class=
=3D"footerSocialListLink" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha0hLdp1BAjEeVOuEOhdqxW=
zLArHsVXws9B1db2dGYRGyMpuC2oNcDN11ceWYZz7FeUw=3D" target=3D"_blank" style=
=3D"font-weight: bold; color: #000000; text-decoration: underline; display:=
 block;"
> <img class=3D"footerSocialListImg" src=3D"https://info.sartorius.com/rs/6=
09-TDQ-677/images/linkedin-icon.png" alt=3D"LinkedIn" style=3D"vertical-ali=
gn: middle; border: 0; outline: none; -ms-interpolation-mode: bicubic; widt=
h: 40px;" width=3D"40" /> </a></td>=20
<td class=3D"footerSocialListItemSpacerHor" style=3D"text-align: left; vert=
ical-align: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"4=
0" align=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"footerSocialListItem" style=3D"text-align: left; vertical-alig=
n: top; width: 40px;" width=3D"40" align=3D"left" valign=3D"top"><a class=
=3D"footerSocialListLink" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha50kY7DIhAe7fUFJbEhxvI=
PJ2duGSNj1qHPjccF-_nL-jlDxPs9HkeeyHXKuS9FBO0c=3D" target=3D"_blank" style=
=3D"font-weight: bold; color: #000000; text-decoration: underline; display:=
 block;"
> <img class=3D"footerSocialListImg" src=3D"https://info.sartorius.com/rs/6=
09-TDQ-677/images/twitter-icon.png" alt=3D"Twitter" style=3D"vertical-align=
: middle; border: 0; outline: none; -ms-interpolation-mode: bicubic; width:=
 40px;" width=3D"40" /> </a></td>=20
<td class=3D"footerSocialListItemSpacerHor" style=3D"text-align: left; vert=
ical-align: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"4=
0" align=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"footerSocialListItem" style=3D"text-align: left; vertical-alig=
n: top; width: 40px;" width=3D"40" align=3D"left" valign=3D"top"><a class=
=3D"footerSocialListLink" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22HaxwgHpQPAsWbvaCsacVclN=
ejXeGBXtm8wEosCcoaivy3f2bmIcQ_wsUNU0Wyczhnhs8=3D" target=3D"_blank" style=
=3D"font-weight: bold; color: #000000; text-decoration: underline; display:=
 block;"
> <img class=3D"footerSocialListImg" src=3D"https://info.sartorius.com/rs/6=
09-TDQ-677/images/youtube-icon.png" alt=3D"YouTube" style=3D"vertical-align=
: middle; border: 0; outline: none; -ms-interpolation-mode: bicubic; width:=
 40px;" width=3D"40" /> </a></td>=20
<td class=3D"footerSocialListItemSpacerHor" style=3D"text-align: left; vert=
ical-align: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"4=
0" align=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"footerSocialListItem" style=3D"text-align: left; vertical-alig=
n: top; width: 40px;" width=3D"40" align=3D"left" valign=3D"top"><a class=
=3D"footerSocialListLink" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha1O-sWHIXCs6t94jAQ7D3l=
wwkpOAd13mCxVuJCnBHL9N5Zzk_L3lolKT5DWkI_G_qr0=3D" target=3D"_blank" style=
=3D"font-weight: bold; color: #000000; text-decoration: underline; display:=
 block;"
> <img class=3D"footerSocialListImg" src=3D"https://info.sartorius.com/rs/6=
09-TDQ-677/images/facebook-icon.png" alt=3D"Facebook" style=3D"vertical-ali=
gn: middle; border: 0; outline: none; -ms-interpolation-mode: bicubic; widt=
h: 40px;" width=3D"40" /> </a></td>=20
</tr>=20
<tr>=20
<td class=3D"footerSocialSpacerBottom" colspan=3D"7" style=3D"text-align: l=
eft; vertical-align: top; height: 20px; font-size: 0px; line-height: 0px;" =
height=3D"20" align=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
</tbody>=20
</table> </th>=20
</tr>=20
</tbody>=20
</table> </td>=20
</tr>=20
=20
<tr>=20
<td style=3D"text-align: left; vertical-align: top;" align=3D"left" valign=
=3D"top">=20
<table class=3D"footerLegal" style=3D"border: none; border-collapse: collap=
se; border-spacing: 0px; cellpadding: 0; cellspacing: 0; role: presentation=
; mso-table-lspace: 0pt; mso-table-rspace: 0pt; width: 515px;" width=3D"515=
" cellpadding=3D"0" cellspacing=3D"0" role=3D"presentation">=20
<tbody>=20
<tr>=20
<td class=3D"globalText footerLegalCol" style=3D"font-family: Arial, sans-s=
erif; font-weight: normal; text-align: left; vertical-align: top; width: 22=
5px; font-size: 12px; line-height: 16px; color: #ffffff;" width=3D"225" ali=
gn=3D"left" valign=3D"top">Published by<br /> Sartorius AG<br /> Otto-Brenn=
er-Stra=C3=9Fe 20<br /> 37079 Goettingen, Germany<br /><br /> Phone +49.551=
.308-0<br /> Fax +49.551.308-3289<br /> <a class=3D"globalLink footerLink" =
href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha8SBy5FCgHmG6PtTk9TTjL=
dx9q0IeRNJJpTRb_pCAh2tlPkTR5UTKHF5HwwvL5bC3Nc=3D" style=3D"text-decoration:=
 underline; font-weight: normal; color: #ffffff;"
>newsletter@sartorius.com<br /> </a><a class=3D"globalLink footerLink" href=
=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha6fpMeRAGSRe8LxYdh0gEG=
-azWMJ5VSTgpvghQiD8i0A9Y1gw9MA7OUztq6WlJ0EJno=3D" style=3D"text-decoration:=
 underline; font-weight: normal; color: #ffffff;"
>www.sartorius.com</a><br /><br /> Executive Board Chairman<br /> Dr. Joach=
im Kreuzburg, Dr. Ren=C3=A9 F=C3=A1ber,<br /> Dr. Alexandra Gatzemeyer,<br =
/> Rainer Lehmann</td>=20
<td class=3D"footerLegalInnerSpacerHor" style=3D"text-align: left; vertical=
-align: top; width: 65px; font-size: 0px; line-height: 0px;" width=3D"65" a=
lign=3D"left" valign=3D"top">&nbsp;</td>=20
<td class=3D"globalText footerLegalCol" style=3D"font-family: Arial, sans-s=
erif; font-weight: normal; text-align: left; vertical-align: top; width: 22=
5px; font-size: 12px; line-height: 16px; color: #ffffff;" width=3D"225" ali=
gn=3D"left" valign=3D"top">Chairman of the Supervisory Board:<br /> Dr. Lot=
har Kappich<br /> Registered Office:<br /> Goettingen, Germany<br /><br /> =
Local Court of Registration:<br /> Amtsgericht Goettingen, HRB No. 1970<br =
/> Vat No. DE 115 302 222<br /><br /> <strong>Unsubscribe newsletter or upd=
ate profile data<br /> If you do not wish to receive<br /> any more informa=
tion from Sartorius or update your profile data,<br /> please <a class=3D"g=
lobalLink footerLink" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha3mAYW7uyDUyf_VmX_9hvC=
Ya_YZsmpIAKlj1hbVAgRWUxsPEiRgZC-i-1HEt9fhEkuk=3D" style=3D"text-decoration:=
 underline; font-weight: normal; color: #ffffff;"
>click here. </a></strong><br /><br /><strong>Further information on the pr=
ocessing of personal data can be found in the <a class=3D"globalLink footer=
Link" href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha_vLrR40KZNTbI8oCX9qru=
T5kQOV9AK1oamkarrxIeRD5NwjFG03X-e4RMuLS1UD58I=3D" style=3D"text-decoration:=
 underline; font-weight: normal; color: #ffffff;"
>Sartorius Data Privacy Notice.</a></strong></td>=20
</tr>=20
</tbody>=20
</table> </td>=20
</tr>=20
=20
</tbody>=20
</table> </td>=20
<td class=3D"globalModuleSpacerHor" style=3D"text-align: left; vertical-ali=
gn: top; width: 40px; font-size: 0px; line-height: 0px;" width=3D"40" align=
=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
<tr>=20
<td class=3D"footerInnerSpacerBottom" colspan=3D"3" style=3D"text-align: le=
ft; vertical-align: top; font-size: 0px; line-height: 0px; height: 30px;" h=
eight=3D"30" align=3D"left" valign=3D"top">&nbsp;</td>=20
</tr>=20
</tbody>
</table> </td>=20
</tr>=20
</tbody>
</table> </td>=20
</tr>=20
</tbody>
</table> </td>=20
</tr>=20
</tbody>
</table> =20
<a href=3D
"https://click.sartorius.com/NjA5LVREUS02NzcAAAGMh22Ha7uXfRiRnmyjnYEK4xpUzH=
lhmmdGlHRSuypknVyO1lx9R5oZNljUZ48I-qLzgQQh2U8=3D"
></a>
<img src=3D"https://click.sartorius.com/trk?t=3D1&mid=3DNjA5LVREUS02Nzc6MDo=
yODk2MToxNDI3MzQ6ODA5NTExOjIxNTg2Ojk6MzM5MzQ2OjQzMTE0Nzc6cmNydXpAc2FwYXNlcX=
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