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Subject: Download: Maximize NK Cell Manufacturing Efficiency with LabX
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DOWNLOAD FREE APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VVNqHm6BFYtBW5cxn5=
g7YX6RNW76My975cfsxwN2nfYwx5m_5PW50kH_H6lZ3pcW2kbSJ67gYDzhW4Y0GGL7gzD01W5m6=
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2ckr8W4gxdf31JbGg04 )

Optimization and scale-up of genetically modified NK cells

Natural killer (NK) cells are part of the innate immune system and have bee=
n shown to be responsible for rapidly identifying and killing foreign, vira=
lly infected, and tumorigenic cells. Like T cells, NK cells can be genetica=
lly modified to increase specificity and potency for cell therapy applicati=
ons. However, compared to T cells, genetic modification of primary NK cells=
 has proven significantly more difficult.

This application note details screening and optimization experiments using =
the Gibco CTS Rotea Counterflow Centrifugation System and the Gibco CTS Xen=
on Electroporation System for upstream and downstream cell processing and g=
ene editing. The scalability and efficiency of these systems for geneticall=
y-modified NK cell therapy manufacturing are investigated.

Key points:

- Electroporation with Cas9 RNP followed by adeno-associated virus transduc=
tion provides efficient and stable editing of NK cells.
- The Gibco CTS Rotea Counterflow Centrifugation System and CTS Xenon Elect=
roporation System are effective tools for upstream and downstream cell proc=
essing and payload delivery.
- The CTS suite of products is a powerful platform that can further the eff=
orts toward creating an efficient NK cell therapy manufacturing process.

Download this application note (https://email.labxmediagroup.com/e3t/Ctc/LT=
+113/c3tLW04/VVNqHm6BFYtBW5cxn5g7YX6RNW76My975cfsxwN2nfYwx5m_5PW50kH_H6lZ3p=
cW2kbSJ67gYDzhW4Y0GGL7gzD01W5m6R_16QQg3dW3g81-v5PpTw4W6LwFHq7MCT-8W2h4KcD7F=
Ml5jW29f6Qw1vK1h7W2srGl270cNp7W4X6J2z6s41TgW5LgbSy3ptNlQW85HT616mNdy3W6292S=
Y2M80GFW5FvRsW18qnxGW7vrpr-6PNhyYW60L18H6PV83qW8clVC041Yc-yW87fSD44rbgBNW4G=
4WmY5xx8ywW6l3tNJ6GM1gnW8hG80R9ly1M1VZz4S-8lHbltW4zNDp97-4V12W1fBdnG49dc_MW=
8j5n5q3TLx1JW9cf0sX7G7P0fW8z4mJ561_HvdW6x7yl83x_zv6W67MMlC4TLp7DW2QvVVm1VNN=
25W4K133B6qcTk2W7nz5HS1DkN-VW332ckr8W4gxdf31JbGg04 ) to learn about overcom=
ing the challenges of genetically modifying natural killer (NK) cells for c=
ell therapy applications

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play:block" data-hs-link-id=3D"2">
          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">DOWNLOAD FREE APPLICATION NOTE</strong>
        </a>
      </td>
    </tr>
  </tbody></table></div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Optimization and scale-up of genetically modified NK cells</span=
></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Natural killer (NK) cells are part of the innate immune system and ha=
ve been shown to be responsible for rapidly identifying and killing foreign=
, virally infected, and tumorigenic cells. Like T cells, NK cells can be ge=
netically modified to increase specificity and potency for cell therapy app=
lications. However, compared to T cells, genetic modification of primary NK=
 cells has proven significantly more difficult.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">This application note details screening and optimization experiments =
using the Gibco CTS Rotea Counterflow Centrifugation System and the Gibco C=
TS Xenon Electroporation System for upstream and downstream cell processing=
 and gene editing. The scalability and efficiency of these systems for gene=
tically-modified NK cell therapy manufacturing are investigated.</span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%;=
 font-weight:bold"><span style=3D"color: #000000;"><span style=3D"font-fami=
ly: Arial, sans-serif;">Key points:</span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Electroporation with Cas9 RNP followed by adeno-associated virus tra=
nsduction provides efficient and stable editing of NK cells.</span></span><=
/li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The Gibco CTS Rotea Counterflow Centrifugation System and CTS Xenon =
Electroporation System are effective tools for upstream and downstream cell=
 processing and payload delivery.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The CTS suite of products is a powerful platform that can further th=
e efforts toward creating an efficient NK cell therapy manufacturing proces=
s.</span></span></li>
</ul>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VV=
NqHm6BFYtBW5cxn5g7YX6RNW76My975cfsxwN2nfYwx5nR32W50kH_H6lZ3pSVlC1XZ3YYzgFW6=
1mtH14nZ6N5W7rCDQQ2-18qzW7q62LR5_46WdW1M7G4v5-lBC2W5QQNVQ9kPZhkW4-dq4K6rSSR=
KW9869_c1kWfvcW5HWyl08QsHzLW1WPG4G7yNg7gW7Pfnv56qlxBzN7qzS2QXgPVPVnL7d58VmR=
zyW3Pxw4D5zj4B2W1yFRCL5DLdCHW6cqSnV6L3X2NW3yf0VL80v4m2W2ld_D05fMn26W6kwq245=
sqDbMW7s8CJ_3Tvg-JN6z4VnmnjqttW2-g_Mj54v5cpN8F_q5Pp1Sv6N5KYhTpbSzc4W4dgSvR2=
zKxD5N8T4GxKVHtx0N3FMryBQ_ds2VCK1fT5QKcD-W7f0Cjr4qrfJHW4mZ7f47zJjxSW45dKk55=
XD_x1W624BRj5RS5smf3GG7kq04" rel=3D"noopener" style=3D"mso-line-height-rule=
:exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">Download t=
his application note</a> to learn about overcoming the challenges of geneti=
cally modifying natural killer (NK) cells for cell therapy applications</sp=
an></span></p></div></div></td></tr></tbody></table>
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