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Subject: Free Application Note From Thermo Fisher Scientific: Gibco CTS
 Rotea Counterflow Centrifugation System
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DOWNLOAD FREE APPLICATION NOTE
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VWzzWp61Qhs7W8mfr8=
z2vCND4W5yX2x95gtbsWN84392q5m_5PW50kH_H6lZ3mXW7Kkd5G6BfM1VW67pPxG88YgJzW91c=
z9V88d7LXW8B57hV4gXTRQW857-lt3FjHr4W5zMrb83smLjDW5Z_sbW18l7BGW3Cw1kD8hTx9JW=
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hb8y_zq6f2LbL7P04 )

Optimization and scale-up of genetically modified NK cells

Natural killer (NK) cells are part of the innate immune system and have bee=
n shown to be responsible for rapidly identifying and killing foreign, vira=
lly infected, and tumorigenic cells. Like T cells, NK cells can be genetica=
lly modified to increase specificity and potency for cell therapy applicati=
ons. However, compared to T cells, genetic modification of primary NK cells=
 has proven significantly more difficult.

This application note details screening and optimization experiments using =
the Gibco CTS Rotea Counterflow Centrifugation System and the Gibco CTS Xen=
on Electroporation System for upstream and downstream cell processing and g=
ene editing. The scalability and efficiency of these systems for geneticall=
y-modified NK cell therapy manufacturing are investigated.

Key points:

- Electroporation with Cas9 RNP followed by adeno-associated virus transduc=
tion provides efficient and stable editing of NK cells.
- The Gibco CTS Rotea Counterflow Centrifugation System and CTS Xenon Elect=
roporation System are effective tools for upstream and downstream cell proc=
essing and payload delivery.
- The CTS suite of products is a powerful platform that can further the eff=
orts toward creating an efficient NK cell therapy manufacturing process.

Download this application note (https://email.labxmediagroup.com/e3t/Ctc/LT=
+113/c3tLW04/VWzzWp61Qhs7W8mfr8z2vCND4W5yX2x95gtbsWN84392q5m_5PW50kH_H6lZ3m=
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LW8Tjnxp2nLs5BV4Z11J5_dJwQW8Mb_hb8y_zq6f2LbL7P04 ) to learn about overcomin=
g the challenges of genetically modifying natural killer (NK) cells for cel=
l therapy applications

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Z3mrW3cCFP55mWtJVN4T8nnwcMYDlW1gVC-f2njxncW4t81ss9kNxmkW1PSrZ461cMCYW6vSFKd=
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          <strong style=3D"color:#ffffff;font-weight:normal;text-decoration=
:none;font-style:normal;">DOWNLOAD FREE APPLICATION NOTE</strong>
        </a>
      </td>
    </tr>
  </tbody></table></div></td></tr></tbody></table>
<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">Optimization and scale-up of genetically modified NK cells</span=
></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Natural killer (NK) cells are part of the innate immune system and ha=
ve been shown to be responsible for rapidly identifying and killing foreign=
, virally infected, and tumorigenic cells. Like T cells, NK cells can be ge=
netically modified to increase specificity and potency for cell therapy app=
lications. However, compared to T cells, genetic modification of primary NK=
 cells has proven significantly more difficult.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">This application note details screening and optimization experiments =
using the Gibco CTS Rotea Counterflow Centrifugation System and the Gibco C=
TS Xenon Electroporation System for upstream and downstream cell processing=
 and gene editing. The scalability and efficiency of these systems for gene=
tically-modified NK cell therapy manufacturing are investigated.</span></sp=
an></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%;=
 font-weight:bold"><span style=3D"color: #000000;"><span style=3D"font-fami=
ly: Arial, sans-serif;">Key points:</span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Electroporation with Cas9 RNP followed by adeno-associated virus tra=
nsduction provides efficient and stable editing of NK cells.</span></span><=
/li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The Gibco CTS Rotea Counterflow Centrifugation System and CTS Xenon =
Electroporation System are effective tools for upstream and downstream cell=
 processing and payload delivery.</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">The CTS suite of products is a powerful platform that can further th=
e efforts toward creating an efficient NK cell therapy manufacturing proces=
s.</span></span></li>
</ul>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;"><a href=3D"https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VW=
zzWp61Qhs7W8mfr8z2vCND4W5yX2x95gtbsWN84392q5nR32W50kH_H6lZ3mPM3dMgS3Y9XbW8v=
5cVm289mbgW28NF8q6gtwlrN5_DMG-5nrYJW2dk64P4Jj70_W97gD8D9dqGQpW4t6qxv1CHJbgW=
7lPNwt3YKgrDW2sTblR9f_KNcW1lfW-B1wGmGcW6r5HS17Yr7VwN5yHwttHx0MyN9gfcqnMZwWm=
N3BFw8dY_DMHW88VlwR3tbB3gW1Y5Wx25CDvcCW4J3tyg2zYG1jW31Z6gJ2Y5c6SW7ZtpK26F14=
2zW1W4Y4R38rb00V-CbNB2lsjWZW1c3JsW3W_ZytW2mN2V-2X1LXqW3pdKJm1FmvPnW1XtYz53x=
px7dW1Mksb95sh6JpW2lh_y47psZK5N11M9PHTlznRVnjmXJ7wRm7fW86lSQK3MWRghW56cF9j8=
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exactly; color:#929eb2" data-hs-link-id=3D"3" target=3D"_blank">Download th=
is application note</a> to learn about overcoming the challenges of genetic=
ally modifying natural killer (NK) cells for cell therapy applications</spa=
n></span></p></div></div></td></tr></tbody></table>
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