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Subject: Download: Unlock Faster, More Efficient HPLC Workflows
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A Helpful Guide to Modernizing Your Old HPLC Methods

Modernizing older or compendial methods can help increase your lab=E2=80=99=
s productivity and lower your cost and carbon footprint. This guide gives y=
ou an overview of what method parameters to consider changing, as defined b=
y USP 621.

Important considerations include:

- Switching from HPLC to UHPLC
- Column particle size
- Column length and inner diameter
- Column temperature and backpressure
- Flow rates, injection volumes, and detector settings

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PW1jgrrW4kK5znW3-Dh235Jk6kQW38L53Z9bY8QTW5rWKvd7XZKg1W7pmCqb32b7PGW7v5R7j7q=
hnK0W9815rr3nCvyfW45BH055MS4CZW2kQx0s33kSRKW827-1W2vPBxgW1BCX0451f-HMN280Kp=
CkkFqPW51tJ6l4zJ6L9W12RZCq6q3GpYW8gKqn03NjwMZW2JcvtX6cBYJ0W708y1x7D1bzWW67L=
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ame=3Dlabx%20red%20lgogo-png.png 150w, https://viewonline.labx.com/hs-fs/hu=
bfs/1.1-%20All-Images/Allan%20Utton/labx%20red%20lgogo-png.png?upscale=3Dtr=
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hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: inherit; =
font-size: inherit; line-height: inherit;" data-hs-cos-general-type=3D"widg=
et" data-hs-cos-type=3D"module"><table class=3D"hse-image-wrapper" role=3D"=
presentation" width=3D"100%" cellpadding=3D"0" cellspacing=3D"0" style=3D"b=
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; mso-line-height-rule:exactly; font-family:Georgia, serif; color:#23286b; =
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VVY3k01bvRSGW4pWZlg3X05XsW2C4PM95gw35dN4KxWNs3qgyTW95jsWP6lZ3nwW6w8fjN1d_f0=
8W5YDgX13VRBnKW6nhgRw8wH_tXW6LkK7w217zGtN73Wwqq52j-5W7FYzfv7G5c8xW59ZwG68Yt=
_TTW8Kr17H3z8Z_JW8MHkPl3VT3NPMlCKwx3cPyXW453CLb18RZGSW5k9xW77S_r3PW3w3Z734V=
cc0BW8S3JzN7Wrsf8W190RCn4Kbb__W5cHcfx7cz4tPW3G1sB31pP_8vW5Cv-4Y8G58FgW4gGN9=
B4QdC0fW359vwZ1cqwPBW98J3v853V89pW7Mmh3J4pKtMXW97TNt686k8vGVQ4mJv21KS5kW5FW=
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tly" data-hs-link-id=3D"1">
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adding-right:10px">
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8px" bgcolor=3D"#FFFFFF">
    
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table-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" =
style=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-famil=
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          <td align=3D"center" valign=3D"middle" bgcolor=3D"#D2232B" style=
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3y93d-4gDW7J2zK18nKHggN2D4YYNTWpw5W5XVLBs5K8LZRW1NN5NZ6ncrpsW21BGbK8mMsypW4=
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<table role=3D"presentation" cellpadding=3D"0" cellspacing=3D"0" width=3D"1=
00%" style=3D"border-spacing:0 !important; border-collapse:collapse; mso-ta=
ble-lspace:0pt; mso-table-rspace:0pt"><tbody><tr><td class=3D"hs_padded" st=
yle=3D"border-collapse:collapse; mso-line-height-rule:exactly; font-family:=
Georgia, serif; font-size:18px; color:#23286b; word-break:break-word; paddi=
ng:10px 20px"><div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_=
cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"colo=
r: inherit; font-size: inherit; line-height: inherit;" data-hs-cos-general-=
type=3D"widget" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_modul=
e_16242852582431_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wra=
pper_type_rich_text" style=3D"color: inherit; font-size: inherit; line-heig=
ht: inherit;" data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_=
text"><h1 style=3D"margin:0; mso-line-height-rule:exactly; font-family:Geor=
gia, serif; color:#23286b; font-size:18px; line-height:125%; text-align:cen=
ter" align=3D"center"><span style=3D"color: #000000; font-family: Arial, sa=
ns-serif;">A Helpful Guide to Modernizing Your Old HPLC Methods</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Modernizing older or compendial methods can help increase your lab=E2=
=80=99s productivity and lower your cost and carbon footprint. This guide g=
ives you an overview of what method parameters to consider changing, as def=
ined by USP 621.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">Important considerations include:</span></span></p>
<ul style=3D"mso-line-height-rule:exactly; line-height:175%; list-style-typ=
e:circle">
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Switching from HPLC to UHPLC</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Column particle size</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Column length and inner diameter</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Column temperature and backpressure</span></span></li>
<li style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%=
"><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-s=
erif;">Flow rates, injection volumes, and detector settings</span></span></=
li>
</ul>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%;=
 text-align:center" align=3D"center"><a href=3D"https://email.labxmediagrou=
p.com/e3t/Ctc/LT+113/c3tLW04/VVY3k01bvRSGW4pWZlg3X05XsW2C4PM95gw35dN4KxWNs3=
qgyTW95jsWP6lZ3mYW5h8xJL8Bx532W8g6Wmw8DRTgBW8tC1yC2z4T7ZW7FdzhJ4xjCzTW8VBZr=
n6_GWPJW1py7n31wq09zN6psh506d16-W7f2_G38s760XW7pQ8Rs8h5Q14N4sp7kmsTDHSMPyVT=
zKdtrlW2x2qkH8xqFhpW1VMPKq4vJ4KCW97-WZ96_PX7XW8XR48z6Cg-BqW18tZmN2SM9n1W7VC=
BWM5BxjKNW16ktD-6Jsh-xW8QLJXd8J5R1VW2K0TDL7dvWRjW72P-vR6Q5dL9W1M81vj4nYzGXW=
4l2jtQ2YgC78W7VwsQ32Dw9bCW6cZY6v34d8KRW7LQVb_78mW-qW87KhXH6qfY71W7lbc5P6pm2=
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<span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-ser=
if;">Download the free guide to learn more </span></span></a></p></div></di=
v></td></tr></tbody></table>
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