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Subject: Download: The benefits of polymer-based reverse-phase resin for
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Download Application Note
(https://email.labxmediagroup.com/e3t/Ctc/LT+113/c3tLW04/VW4t3v40hBnkW9bL0_=
H69mWm6W8BHpjy5kCxB8N1WCS0b3prCCW95jsWP6lZ3lnW7YlSkZ66wTRdW2Kq0Bf8d0WtWW8BK=
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21n4ymW6kYtqq6d6-42N15zycCPh8KDW9bmLKl1JJrHKVnnpy09kqdJNf8zg7xR04 )

The benefits of polymer-based reverse-phase resin for isolation of key GPCR=
 binding peptides

The G protein-coupled receptor (GPCR) has shown significance in the develop=
ment of new therapeutic treatments. Analysis of peptides in the gastrointes=
tinal (GI) tract that bind to GPCRs has led to discoveries that relate thes=
e peptides to other functional systems like the endocrine, cardiac, and cen=
tral nervous systems. A key aspect in linking peptide relationships is foun=
d in the purification and identification of peptides in various systems. Is=
olation of these peptides may help identify possible metabolic changes that=
 take place leading to endocrine disorders such as thyroid disease or diabe=
tes, cell proliferation, and kidney disease.

This application note examines use of the polymer-based reversed-phase resi=
n (PRP-3) for the successful chromatographic separation of seven key GPCR b=
inding peptides. The conditions and performance characteristics are describ=
ed as well as the putative mechanism underlying isolation.

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00%"><tbody><tr><td class=3D"hs_padded" style=3D"font-family:Georgia, serif=
; font-size:18px; color:#23286b; word-break:break-word; padding:10px 20px">=
<div id=3D"hs_cos_wrapper_module_16242852582431" class=3D"hs_cos_wrapper hs=
_cos_wrapper_widget hs_cos_wrapper_type_module" style=3D"color: inherit; fo=
nt-size: inherit; line-height: inherit;" data-hs-cos-general-type=3D"widget=
" data-hs-cos-type=3D"module"><div id=3D"hs_cos_wrapper_module_162428525824=
31_" class=3D"hs_cos_wrapper hs_cos_wrapper_widget hs_cos_wrapper_type_rich=
_text" style=3D"color: inherit; font-size: inherit; line-height: inherit;" =
data-hs-cos-general-type=3D"widget" data-hs-cos-type=3D"rich_text"><h1 styl=
e=3D"margin:0; mso-line-height-rule:exactly; font-family:Georgia, serif; co=
lor:#23286b; font-size:18px; line-height:125%; text-align:center" align=3D"=
center"><span style=3D"color: #000000; font-family: Arial, sans-serif;">The=
 benefits of polymer-based reverse-phase resin for isolation of key GPCR bi=
nding peptides</span></h1>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">The G protein-coupled receptor (GPCR) has shown significance in the d=
evelopment of new therapeutic treatments. Analysis of peptides in the gastr=
ointestinal (GI) tract that bind to GPCRs has led to discoveries that relat=
e these peptides to other functional systems like the endocrine, cardiac, a=
nd central nervous systems. A key aspect in linking peptide relationships i=
s found in the purification and identification of peptides in various syste=
ms. Isolation of these peptides may help identify possible metabolic change=
s that take place leading to endocrine disorders such as thyroid disease or=
 diabetes, cell proliferation, and kidney disease.</span></span></p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
>&nbsp;</p>
<p style=3D"mso-line-height-rule:exactly; font-size:15px; line-height:125%"=
><span style=3D"color: #000000;"><span style=3D"font-family: Arial, sans-se=
rif;">This application note examines use of the polymer-based reversed-phas=
e resin (PRP-3) for the successful chromatographic separation of seven key =
GPCR binding peptides. The conditions and performance characteristics are d=
escribed as well as the putative mechanism underlying isolation. </span></s=
pan></p></div></div></td></tr></tbody></table>
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